ZFIN ID: ZDB-FIG-190723-1596
Chen et al., 2019 - Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition. PLoS Genetics   15:e1008058 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Bud

Fig 5 Klf4 maintains epidermal stem cell proliferation by repression of <italic>p53</italic> expression.

(A) BrdU and p63 labeling were performed on embryos injected with klf4 5mmMO2 (control) (a-e), combined klf4 MO1/klf4 MO2 (f), p53 MO (g), combined cdkn1a MO/ klf4 MO1/klf4 MO2 (h), combined p53 MO/klf4 MO1/klf4 MO2 (i), combined klf4-7mm mRNA/klf4 MO1/klf4 MO2 (j), or combined klf4ΔC-7mm mRNA/klf4 MO1/klf4 MO2 (k). Nuclei are counterstained with Hoechst 33342 (c). Both p63+ and p63+BrdU+ cell number were enumerated in the circled areas. Examples of p63 and BrdU colocalized cells (asterisk) are shown. The percentage of p63+BrdU+ cells in embryos after indicated treatments was quantified (l). (B) p53 expression in embryos injected with combined klf4 MO1/klf4 MO2, combined klf4-7mm mRNA/klf4 MO1/klf4 MO2, or combined klf4ΔC-7mm mRNA/klf4 MO1/klf4 MO2 was measured by RT-qPCR. (C) cdkn1a/p21 expression in embryos after indicated treatments was measured by RT-qPCR. Statistical significance was determined by Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bar, 50 μm. Error bars indicate standard error.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tp63 WT + MO1-cdkn1a + MO1-klf4 + MO2-klf4 standard conditions Bud ectoderm somatic stem cell IHC
WT + MO1-klf4 + MO2-klf4 standard conditions Bud ectoderm somatic stem cell IHC
WT + MO1-klf4 + MO2-klf4 + MO4-tp53 standard conditions Bud ectoderm somatic stem cell IHC
WT + MO2-klf4 control Bud ectoderm somatic stem cell IHC
WT + MO4-tp53 standard conditions Bud ectoderm somatic stem cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdkn1a + MO1-klf4 + MO2-klf4 standard conditions Bud ectoderm cell population proliferation occurrence, ameliorated
WT + MO1-klf4 + MO2-klf4 standard conditions Bud ectoderm stem cell proliferation decreased occurrence, abnormal
WT + MO1-klf4 + MO2-klf4 + MO4-tp53 standard conditions Bud ectoderm cell population proliferation occurrence, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.