ZFIN ID: ZDB-FIG-190604-19
Ahmed Alfar et al., 2017 - Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity. Scientific Reports   7:3994 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 1

Catalase expression in beta-cells leads to a reduction in beta-cell numbers in larvae. (A) Whole mount in situ hybridization (WISH) for catalase in WT and Tg(ins:catalase;ins:H2BmCherry) larvae at 5 dpf. Arrows point to the principal islet. Catalase expression was not detected in the beta-cells of WT larvae whereas Tg(ins:catalase;ins:H2BmCherry) larvae exhibit catalase transcripts. (B) Double WISH for insulin (brown) and catalase (purple) showing overlap between the insulin and catalase transcripts in Tg(ins:catalase;ins:H2BmCherry) siblings. (C) Confocal projections of the principal islets of 4 dpf WT and Tg(ins:catalase;ins:H2BmCherry) larvae. (D) Quantification of the average number of beta-cell in WT (n = 15 larvae) and Tg(ins:catalase;ins:H2BmCherry) larvae (n = 12 larvae). Tg(ins:catalase;ins:H2BmCherry) larvae exhibit a reduced beta-cell number compared to WT (p = 0,008, error bars = SEM).

 

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cat WT standard conditions Day 5 liver ISH
tud206Tg standard conditions Day 5 liver ISH
Day 5 primary islet ISH
ins WT standard conditions Day 5 primary islet ISH
tud206Tg standard conditions Day 4 primary islet ISH
Day 5 primary islet ISH
mCherry tud206Tg standard conditions Day 4 pancreatic B cell IHC
Day 4 primary islet IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tud206Tg standard conditions Day 4 pancreatic B cell decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.