ZFIN ID: ZDB-FIG-190604-21
Ahmed Alfar et al., 2017 - Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity. Scientific Reports   7:3994 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 4

Different levels of H2O2 induce beta-cell proliferation and quiescence. (A) Quantification of the number of Tg(ins:FUCCI-S/G2/M)+ beta-cells after continuous treatment with increasing concentrations of H2O2 from 3 to 4 dpf. The treatment with 50 μM H2O2 (n = 89 larvae) increased the number of Tg(ins:FUCCI-S/G2/M)+ beta-cells compared to controls (n = 104 larvae) (p = 0.025) whereas the treatments with 500 and 1000 μM H2O2 decreased the number of Tg(ins:FUCCI-S/G2/M)+ beta-cells (n = 38 larvae for 500 H2O2 μM; n = 63 larvae for controls; p = 0.03) (n = 46 larvae for 1000 μM H2O2; n = 78 larvae for controls; p = 0.0004). (B) Quantification of the number of Tg(ins:FUCCI-S/G2/M)+ beta-cells in 3 dpf larvae treated with increasing concentrations of H2O2 for 1.5 hours followed by a wash-out. The number of Tg(ins:FUCCI-S/G2/M)+ beta-cells was examined 24 hours after the initiation of the treatment. The short treatment with 200 and 500 μM H2O2 increased the number of Tg(ins:FUCCI-S/G2/M)+ beta-cells compared to controls (n = 51 larvae for 200 μM H2O2; n = 86 larvae for controls; p = 0.02) (n = 68 larvae for 500 μM H2O2; n = 96 for larvae for controls; p = 0.02). (C) Confocal projections of the principal islets of 4 dpf Tg(ins:H2B-GFP; ins:dsRED) larvae treated from 3 to 4 dpf with 50 μM H2O2. (D) Quantification of the average number of beta-cells marked with H2B-GFP in controls and H2O2-treated larvae. H2O2-treatement increased beta-cell number (n = 30 larvae) compared to controls (n = 26 larvae) (p = 0,046). Error bars = SEM. (E) Bar chart showing the increase in the number of INS-1 cells after H2O2 or NAC-treatments. Data from four independent experiments and > 3.5 × 105 cells per condition were normalized to the mean of control treated cells ( = 100%) and analyzed for significance using a one-way ANOVA with Dunnett’s multiple comparison test. Error bars indicate S.E.M. Cartoons with permission by Luis Delgadillo.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s960Tg chemical treatment by environment: hydrogen peroxide Day 4 pancreatic B cell IFL
mAGFP s947Tg chemical treatment by environment: hydrogen peroxide Protruding-mouth to Day 4 pancreatic B cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s947Tg chemical treatment by environment: hydrogen peroxide Protruding-mouth - Day 4 pancreatic B cell cell population proliferation occurrence, abnormal
Protruding-mouth - Day 4 pancreatic B cell mAGFP expression decreased amount, abnormal
Protruding-mouth - Day 4 pancreatic B cell mAGFP expression increased amount, abnormal
s960Tg chemical treatment by environment: hydrogen peroxide Day 4 pancreatic B cell increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.