ZFIN ID: ZDB-FIG-190604-20
Ahmed Alfar et al., 2017 - Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity. Scientific Reports   7:3994 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Days 21-29
PHENOTYPE:
Fish:
Observed In:
Stage: Days 21-29

Fig. 2

Catalase expression in beta-cells reduces beta-cell proliferation. (A) Confocal sections of principal islets from WT and Tg(ins:catalase;ins:H2BmCherry) siblings, incubated at 21 dpf with EdU for 16 hours (5 h after feeding). Fewer beta-cells incorporated EdU in Tg(ins:catalase;ins:H2BmCherry) compared to WT siblings. (B) Quantification of the percentage of EdU+ beta-cells in WT (n = 10) and Tg(ins:catalase;ins:H2BmCherry) (n = 11). Expression of catalase leads to a reduced percentage of EdU+ beta-cells compared to WT (p = 0,001). (C) Confocal planes (xzy) and 3D reconstructions (xzy) of smaller islets from WT and Tg(ins:catalase;ins:H2BmCherry) animals stained for insulin and glucagon. The beta-cells in the islet of the Tg(ins:catalase;ins:H2BmCherry) were scattered and fewer in numbers compared to WT. Alpha-cells in Tg(ins:catalase;ins:H2BmCherry) appear unaffected by the catalase expression in beta-cells. See also Figure S2 for maximum projections spanning the volume of principal islets in WT and Tg(ins:catalase;ins:H2BmCherry). Error bars = SEM.

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry tud206Tg standard conditions Days 21-29 pancreatic B cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tud206Tg standard conditions Days 21-29 pancreatic A cell amount, normal
Days 21-29 pancreatic B cell cell population proliferation decreased occurrence, abnormal
Days 21-29 pancreatic B cell decreased amount, abnormal
Days 21-29 primary islet pancreatic B cell distributed, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.