Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity
- Ahmed Alfar, E., Kirova, D., Konantz, J., Birke, S., Mansfeld, J., Ninov, N.
- Scientific Reports 7: 3994 (Journal)
- Registered Authors
- Birke, Sarah, Ninov, Nikolay
- MeSH Terms
- Animals, Genetically Modified
- Cell Line
- Cell Plasticity*
- Cell Proliferation
- Hydrogen Peroxide/metabolism
- Insulin-Secreting Cells/metabolism*
- Reactive Oxygen Species*
- 28652605 Full text @ Sci. Rep.
Ahmed Alfar, E., Kirova, D., Konantz, J., Birke, S., Mansfeld, J., Ninov, N. (2017) Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity. Scientific Reports. 7:3994.
The pancreatic beta-cells control glucose homeostasis by secreting insulin in response to nutrient intake. The number of beta-cells is under tight metabolic control, as this number increases with higher nutrient intake. However, the signaling pathways matching nutrition with beta-cell mass plasticity remain poorly defined. By applying pharmacological and genetic manipulations, we show that reactive oxygen species (ROS) regulate dose-dependently beta-cell proliferation in vivo and in vitro. In particular, reducing ROS levels in beta-cells blocks their proliferation in response to nutrients. Using a non-invasive genetic sensor of intracellular hydrogen peroxide (H2O2), we reveal that glucose can directly increase the levels of H2O2. Furthermore, a moderate increase in H2O2 levels can stimulate beta-cell proliferation. Interestingly, while high H2O2 levels are inhibitory to beta-cell proliferation, they expand beta-cell mass in vivo by inducing rapid beta-cell neogenesis. Our study thus reveals a ROS-level-dependent mechanism linking nutrients with beta-cell mass plasticity. Hence, given the requirement of ROS for beta-cell mass expansion, antioxidant therapies should be applied with caution in diabetes.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes