ZFIN ID: ZDB-FIG-180905-21
Genthe et al., 2017 - R-spondin-1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways. Development (Cambridge, England)   144(4):590-600 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 14-19 somites

Fig. 3

Notch pathway gene expression in Rspo1 morphants. Wild-type (A,D,G,J,M,P) or Rspo1 MO-injected animals (B,E,H,K,N,Q) were examined by WISH for expression of the Notch ligands dlc (A,B), dld (D,E), or Notch receptors notch1a (G,H), notch1b (J,K), notch2 (M,N) and notch3 (P,Q) at the time points indicated. Compared with wild-type (A,D), dlc and dld are specifically decreased (B,E) in the formed somites (below red bars), but not the pre-somitic mesoderm. Notch receptor expression is unaffected. Flat mount dorsal views of the trunk or lateral views at 160× magnification. Numbers of embryos displaying the depicted phenotype are indicated. (C,F,I,L,O,R) Histograms illustrate percentages of phenotypes. (S,T) Decreased dlc and dld mRNA in Rspo1 knockdown (Rspo1 MO) was confirmed by qRT-PCR, normalized to expression of ef1a, of transcript isolated from dissected embryo trunks (mean±s.e.m.). *P≤0.0006, determined by one-way ANOVA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlc WT standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
14-19 somites whole organism RTPCR
WT + MO3-rspo1 standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
14-19 somites whole organism RTPCR
dld WT standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
14-19 somites whole organism RTPCR
WT + MO3-rspo1 standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
14-19 somites whole organism RTPCR
notch1a WT standard conditions Prim-5 somite ISH
WT + MO3-rspo1 standard conditions Prim-5 somite ISH
notch1b WT standard conditions Prim-5 somite ISH
WT + MO3-rspo1 standard conditions Prim-5 somite ISH
notch2 WT standard conditions Prim-5 somite ISH
WT + MO3-rspo1 standard conditions Prim-5 somite ISH
notch3 WT standard conditions 14-19 somites somite ISH
WT + MO3-rspo1 standard conditions 14-19 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-rspo1 standard conditions 14-19 somites somite dld expression decreased amount, abnormal
14-19 somites somite dlc expression decreased amount, abnormal
14-19 somites whole organism dld expression decreased amount, abnormal
14-19 somites whole organism dlc expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development