ZFIN ID: ZDB-FIG-180905-25
Genthe et al., 2017 - R-spondin-1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways. Development (Cambridge, England)   144(4):590-600 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 26+ somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 26+ somites to Prim-5

Fig. S2

Rspo1, runx1, wnt16 levels in Rspo1 MO or Wnt16 MO animals. (A) Image of WT embryo at 24 hpf showing area of images taken and labeled with the location of the neural tube (NT, gray), dorsal aorta (DA, red line), posterior cardinal vein (PCV, blue line), and yolk sac (YS). Representative wild type (WT; B,E,H), Rspo1 MO (C,F,I), and Wnt16 MO (D,G,J) embryos processed by WISH for rspo1 (B-D), runx1 (E-G), and wnt16 (H-J) mRNA expression at 22 hpf or 24 hpf as indicated in each image. Lateral views of the trunk at 160x magnification. Numbers of embryos displaying the depicted phenotype are indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
rspo1 WT standard conditions 26+ somites trunk mesoderm ISH
WT + MO2-wnt16 standard conditions 26+ somites trunk mesoderm ISH
WT + MO3-rspo1 standard conditions 26+ somites trunk mesoderm ISH
runx1 WT standard conditions Prim-5 dorsal aorta hematopoietic stem cell ISH
WT + MO2-wnt16 standard conditions Prim-5 Not Detected dorsal aorta hematopoietic stem cell ISH
WT + MO3-rspo1 standard conditions Prim-5 Not Detected dorsal aorta hematopoietic stem cell ISH
wnt16 WT standard conditions Prim-5 somite ISH
WT + MO2-wnt16 standard conditions Prim-5 somite ISH
WT + MO3-rspo1 standard conditions Prim-5 somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-wnt16 standard conditions Prim-5 dorsal aorta hematopoietic stem cell runx1 expression absent, abnormal
Prim-5 dorsal aorta hematopoietic stem cell absent, abnormal
Prim-5 somite wnt16 expression decreased amount, abnormal
WT + MO3-rspo1 standard conditions 26+ somites trunk mesoderm rspo1 expression decreased amount, abnormal
Prim-5 dorsal aorta hematopoietic stem cell runx1 expression absent, abnormal
Prim-5 dorsal aorta hematopoietic stem cell absent, abnormal
Prim-5 somite wnt16 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development