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ZFIN ID: ZDB-FIG-180816-6
Davis et al., 2018 - ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression. Developmental Biology   440(1):40-52 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6

Inducible Etv2 knockdown results in decreased expression of genes associated with lymphangiogenesis as analyzed by in situ hybridization at 56 hpf. (A, E, I) Uninjected larvae illustrate normal flt4, lyve1b and fli1a expression in the PCV. (B, F, J) Larvae injected with photomorph solution and kept in dark show expression pattern similar to uninjected controls. (C, G, K) Larvae injected with photomorph solution and photoactivated at the shield stage (6 hpf) show absent flt4 and lyve1b expression from the major axial vessels. fli1a expression is present in the axial vessels due to partial recovery of Etv2 knockdown phenotype observed at the later stages; however, ISV sprouting is abnormal. (D, H, L) Injected larvae photoactivated at 24 hpf, show decreased flt4 and lyve1b expression (D,H), compared to control larvae. However, note the unaffected pattern of fli1a (L), demonstrating that Etv2 knockdown at 24 hpf specifically affects markers associated with lymphangiogenesis. Analysis for each marker has been repeated at least twice. Number of larvae showing a displayed phenotype out of the total number of larvae is shown in the lower right corner.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fli1a WT control Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec posterior caudal vein IFL
WT standard conditions Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec posterior caudal vein IFL
WT + MO2-etv2 control Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec posterior caudal vein IFL
flt4 WT control Long-pec posterior caudal vein IFL
WT standard conditions Long-pec posterior caudal vein IFL
WT + MO2-etv2 control Long-pec posterior caudal vein IFL
lyve1b WT control Long-pec posterior caudal vein IFL
WT standard conditions Long-pec posterior caudal vein IFL
WT + MO2-etv2 control Long-pec posterior caudal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-etv2 control Long-pec posterior caudal vein lyve1b expression decreased amount, abnormal
Long-pec posterior caudal vein flt4 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 440(1), Davis, J.A., Koenig, A.L., Lubert, A., Chestnut, B., Liu, F., Desai, S.P., Winkler, T., Pociute, K., Choi, K., Sumanas, S., ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression, 40-52, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.