header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180816-1
Davis et al., 2018 - ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression. Developmental Biology   440(1):40-52 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 1

Etv2 expression in wild type embryos and larvae as analyzed by the transgenic reporter expression and in situ hybridization. (A-F) Confocal images of Tg(−2.3 kb etv2:GFP; kdrl:mCherry) reporter line at 24–56 hpf. Note that etv2:GFP expression is downregulated in the dorsal aorta (DA) at 48–56 hpf but is present in multiple cells in the posterior cardinal vein (PCV). (A-C) and (D-F) show the same embryos and larvae in green and overlaid green and red channels. (D′-F′) are higher magnification images of the DA and PCV from embryos and larvae in (D-F). Non-specific expression of etv2:GFP in neural cells is also apparent in (C,F). (G-I) In situ hybridization analysis of etv2 expression at 24–56 hpf. Note the enriched etv2 expression in the PCV (arrowheads) as compared to the DA (arrows) at 48–56 hpf. Each assay has been repeated at least twice with over 10 embryos or larvae analyzed in each group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf372Tg ; ci5Tg standard conditions Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 posterior caudal vein IFL
Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec posterior caudal vein IFL
etv2 WT standard conditions Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior caudal vein ISH
Long-pec dorsal aorta ISH
Long-pec intersegmental vessel ISH
Long-pec posterior caudal vein ISH
mCherry zf372Tg ; ci5Tg standard conditions Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 posterior caudal vein IFL
Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec posterior caudal vein IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 440(1), Davis, J.A., Koenig, A.L., Lubert, A., Chestnut, B., Liu, F., Desai, S.P., Winkler, T., Pociute, K., Choi, K., Sumanas, S., ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression, 40-52, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.