header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180816-5
Davis et al., 2018 - ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression. Developmental Biology   440(1):40-52 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 5

Etv2 depletion by caged MO photoactivation results in reduction of prox1a:GFP+ lymphatic progenitor cells. TgBAC(prox1a:KalT4-UAS:uncTagRFP); Tg(UAS:GFP) live embryos were imaged by confocal microscopy at 32 hpf. The number of prox1a:GFP+ cells is significantly reduced, 1.8 ± 0.32 cells, in embryos where etv2 is depleted at 24 hpf by caged MO photoactivation (n = 12) (B), compared to uninjected embryos (A) (5.1 ± 0.38 cells, n = 13, p < 0.0001, two tailed student's t-test). Arrows indicate prox1a:GFP+ endothelial cells. (C) Quantification of prox1a:GFP positive cells across the width of 9 somites in the trunk region. Mean±SEM is shown. Individual data points are shown in Fig. S4B. This experiment has been replicated twice.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nim5Tg ; nkuasgfp1aTg standard conditions Prim-15 vascular lymphangioblast IFL
nim5Tg ; nkuasgfp1aTg + MO2-etv2 standard conditions Prim-15 vascular lymphangioblast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nim5Tg ; nkuasgfp1aTg + MO2-etv2 standard conditions Prim-15 vascular lymphangioblast decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 440(1), Davis, J.A., Koenig, A.L., Lubert, A., Chestnut, B., Liu, F., Desai, S.P., Winkler, T., Pociute, K., Choi, K., Sumanas, S., ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression, 40-52, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.