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ZFIN ID: ZDB-FIG-180816-4
Davis et al., 2018 - ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression. Developmental Biology   440(1):40-52 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 4

Etv2 depletion by caged MO photoactivation inhibits lymphangiogenesis but not the formation of venous intersegmental vessels (ISVs). (A-D) Analysis of venous ISVs in fli1a:GFP larvae at 4 dpf. (A) Control uninjected larva at 4 dpf, with the thoracic duct (TD) located below the dorsal aorta (DA). Embryos injected with photomorph solution and never photoactivated develop normal TD and vasculature (B), while injected embryos UV photoactivated at 24 hpf (C), develop normal vasculature compared to control embryos, while the thoracic duct is absent (asterisks) at 4 dpf. Note that the venous derived intersegmental vessels are present in both control and Etv2-inhibited larvae (arrowheads). (D) Quantification of percent of ISVs with venous connection at 4 dpf in control uninjected larvae (54.5 ± 1.2%, n = 13) compared to never photoactivated (55.1 ± 1.2%, n = 11, p = 0.74, two tailed Student's t-test) and 24 hpf photoactivated (52.9 ± 1.2%, n = 12, p = 0.34, two tailed Student's t-test) larvae, indicates no significant difference between groups. Mean±SEM is shown. Individual data points are shown in Fig. S4A. This experiment has been replicated 3 times.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP y1Tg control Day 4 dorsal aorta IFL
Day 4 intersegmental vessel IFL
Day 4 thoracic duct IFL
Day 4 trunk vasculature IFL
y1Tg standard conditions Day 4 dorsal aorta IFL
Day 4 intersegmental vessel IFL
Day 4 thoracic duct IFL
Day 4 trunk vasculature IFL
y1Tg + MO2-etv2 standard conditions Day 4 dorsal aorta IFL
Day 4 intersegmental vessel IFL
Day 4 Not Detected thoracic duct IFL
Day 4 trunk vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO2-etv2 standard conditions Day 4 intersegmental vein amount, normal
Day 4 thoracic duct absent, abnormal
Day 4 thoracic duct EGFP expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 440(1), Davis, J.A., Koenig, A.L., Lubert, A., Chestnut, B., Liu, F., Desai, S.P., Winkler, T., Pociute, K., Choi, K., Sumanas, S., ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression, 40-52, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.