ZFIN ID: ZDB-FIG-180302-16
Seritrakul et al., 2017 - Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways. PLoS Genetics   13:e1006987 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 4

Fig 4. Expression of retinal cell fate specification markers is largely normal in tet2-/-;tet3-/- embryos.

Expression of genes involved in retinal neurogenesis was detected by in situ hybridization at 36hpf and 48hpf. (A,F) At 36hpf, vsx2 is expressed in the retinal progenitor cells (RPCs) and turned off as they differentiate, first in the central retina (A, dotted area). This zone of differentiation is still present in tet2-/-;tet3-/- mutant (F, dotted area). (K,P) At 48hpf, expression of vsx2 is slightly expanded in tet2-/-;tet3-/- mutants (K,P arrows) but otherwise appears in a normal pattern. (B,C,G,H,L,M,Q,R) Expression of pax6a (marker for RPC, amacrine, and ganglion cells) and neuroD4 (marker for amacrine, horizontal, and bipolar cells) is relatively normal in tet2-/-;tet3-/- mutants when compared to sibling embryos at both 36hpf and 48hpf. (D,I,N,S) Ganglion cell precursors express atoh7 as they exit the RPC pool, become specified and start undergoing differentiation. atoh7 expression is present in the correct location in tet2-/-;tet3-/- mutants, compared to sibling embryos at both 36hpf and 48hpf. (E,J,O,T) crx is expressed in cells fated to become photoreceptors (rods and cones) and this expression pattern appears relatively normal in tet2-/-;tet3-/- mutants. Scale bar = 20μm. n>8 per gene for each time point. Drawings on bottom row represent cell type detected in each corresponding column.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh7 WT standard conditions Prim-25 retinal ganglion cell ISH
Long-pec retinal ganglion cell ISH
tet2au59/au59 ; tet3au60/au60 standard conditions Prim-25 retinal ganglion cell ISH
Long-pec retinal ganglion cell ISH
crx WT standard conditions Prim-25 photoreceptor cell ISH
Long-pec photoreceptor cell ISH
tet2au59/au59 ; tet3au60/au60 standard conditions Prim-25 photoreceptor cell ISH
Long-pec photoreceptor cell ISH
neurod4 WT standard conditions Prim-25 amacrine cell ISH
Prim-25 horizontal cell ISH
Prim-25 retinal bipolar neuron ISH
Long-pec amacrine cell ISH
Long-pec horizontal cell ISH
Long-pec retinal bipolar neuron ISH
tet2au59/au59 ; tet3au60/au60 standard conditions Prim-25 amacrine cell ISH
Prim-25 horizontal cell ISH
Prim-25 retinal bipolar neuron ISH
Long-pec amacrine cell ISH
Long-pec horizontal cell ISH
Long-pec retinal bipolar neuron ISH
pax6a WT standard conditions Prim-25 amacrine cell ISH
Prim-25 retina ISH
Prim-25 retinal ganglion cell ISH
Long-pec amacrine cell ISH
Long-pec retina ISH
Long-pec retinal ganglion cell ISH
tet2au59/au59 ; tet3au60/au60 standard conditions Prim-25 amacrine cell ISH
Prim-25 retina ISH
Prim-25 retinal ganglion cell ISH
Long-pec amacrine cell ISH
Long-pec retina ISH
Long-pec retinal ganglion cell ISH
vsx2 WT standard conditions Prim-25 retina ISH
Long-pec retina ISH
tet2au59/au59 ; tet3au60/au60 standard conditions Prim-25 retina ISH
Long-pec retina ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tet2au59/au59 ; tet3au60/au60 standard conditions Prim-25 retina vsx2 expression increased distribution, abnormal
Long-pec retina vsx2 expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.