ZFIN ID: ZDB-FIG-180302-15
Seritrakul et al., 2017 - Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways. PLoS Genetics   13:e1006987 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 3

Fig 3. tet2-/-;tet3-/- retinal cells do not undergo terminal differentiation.

(A,F) HuC/D labels RGCs and amacrine cells (ACs), which are reduced in number and located only in the central region of the INL in tet2-/-;tet3-/- retinae (arrow). (B,G) tet2-/-;tet3-/- mutant retinae almost entirely lack zpr-1+ red/green cones (arrow); (C,D,H,I) possess few zpr-3+ rods (arrow), and of those that are zpr-3+, outer segments are severely attenuated or almost absent (arrow). (E,J) tet2-/-;tet3-/- retinae also possess few zrf-1+ Müller glia (arrows in wild-type). In all cases, marker+ cells are located in the central/ventral part of the retina. (K,P) Zn8 detects neurolin, a protein enriched on RGCs and the optic nerve (arrowhead in K). (L,Q) Zn8 staining reveals the optic nerve in the choroid fissure and optic chiasm (arrow) of wild-type embryos but not in tet2-/-;tet3-/- mutants. (M-N) isl2b:GFP transgenics express GFP in RGCs and PRs, clearly labeling the optic nerve in whole-mount and section views (arrowhead). (R,S) The tet2-/-;tet3-/- optic nerve is very thin, often unilaterally formed, but, when present, correctly routed to the brain. (O,T) The isl2b:GFP signal overlaps zpr-3 (rod) marker in the cell body and outer segments in siblings (arrows). In tet2-/-;tet3-/-, few isl2b:GFP+ cells are zpr-3+ (arrows), further suggesting that specified cells are not terminally differentiated. Few outer segments have also formed in tet2-/-;tet3-/- mutants. DNA (blue), antibody stain (red). All images are 3dpf. n>5 for each marker. Dorsal is up and anterior to the left. Scale bar = 80μm in A-K, P.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP tet3au60/au60 ; zc7Tg ; tet2au59/au59 standard conditions Protruding-mouth cranial nerve II IFL
Protruding-mouth photoreceptor cell IHC
Protruding-mouth retinal ganglion cell IFL
Protruding-mouth retinal ganglion cell IHC
zc7Tg standard conditions Protruding-mouth cranial nerve II IHC
Protruding-mouth cranial nerve II IFL
Protruding-mouth photoreceptor cell IHC
Protruding-mouth retinal ganglion cell IHC
Protruding-mouth retinal ganglion cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-elavl IHC WT standard conditions Protruding-mouth amacrine cell
IHC Protruding-mouth retinal ganglion cell
IHC tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retinal ganglion cell
IHC Protruding-mouth retinal inner nuclear layer
zn-8 IHC WT standard conditions Protruding-mouth cranial nerve II
IHC Protruding-mouth retinal ganglion cell
IHC tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retinal ganglion cell
zpr-1 IHC WT standard conditions Protruding-mouth green sensitive photoreceptor cell
IHC Protruding-mouth green sensitive photoreceptor cell photoreceptor outer segment
IHC Protruding-mouth red sensitive photoreceptor cell
IHC Protruding-mouth red sensitive photoreceptor cell photoreceptor outer segment
IHC tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth green sensitive photoreceptor cell
IHC Protruding-mouth Not Detected green sensitive photoreceptor cell photoreceptor outer segment
IHC Protruding-mouth red sensitive photoreceptor cell
IHC Protruding-mouth Not Detected red sensitive photoreceptor cell photoreceptor outer segment
zpr-3 IHC WT standard conditions Protruding-mouth retinal rod cell
IHC Protruding-mouth retinal rod cell photoreceptor outer segment
IHC tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retinal rod cell
IHC Protruding-mouth Not Detected retinal rod cell photoreceptor outer segment
IHC tet3au60/au60 ; zc7Tg ; tet2au59/au59 standard conditions Protruding-mouth retinal rod cell
IHC Protruding-mouth Not Detected retinal rod cell photoreceptor outer segment
IHC zc7Tg standard conditions Protruding-mouth retinal rod cell
IHC Protruding-mouth retinal rod cell photoreceptor outer segment
zrf-1 IHC WT standard conditions Protruding-mouth Muller cell
IHC tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth Muller cell
Phenotype Details
Fish Conditions Stage Phenotype
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth cranial nerve II absent, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth cranial nerve II decreased thickness, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth cranial nerve II axon decreased amount, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth eye decreased size, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth green sensitive photoreceptor cell zpr-1 labeling decreased amount, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth green sensitive photoreceptor cell photoreceptor outer segment zpr-1 labeling absent, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth Muller cell zrf-1 labeling decreased amount, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth red sensitive photoreceptor cell zpr-1 labeling decreased amount, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth red sensitive photoreceptor cell photoreceptor outer segment zpr-1 labeling absent, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retina central region lacks parts or has fewer parts of type retinal cone cell, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retina central region lacks parts or has fewer parts of type retinal rod cell, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retina peripheral region lacks all parts of type retinal cone cell, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retina peripheral region lacks all parts of type retinal rod cell, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retinal ganglion cell cell morphogenesis involved in neuron differentiation decreased occurrence, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retinal rod cell zpr-3 labeling decreased amount, abnormal
tet2au59/au59 ; tet3au60/au60 standard conditions Protruding-mouth retinal rod cell photoreceptor outer segment zpr-3 labeling absent, abnormal
tet3au60/au60 ; zc7Tg ; tet2au59/au59 standard conditions Protruding-mouth cranial nerve II absent, abnormal
Protruding-mouth cranial nerve II decreased thickness, abnormal
Protruding-mouth cranial nerve II axon decreased amount, abnormal
Protruding-mouth retinal rod cell photoreceptor outer segment zpr-3 labeling absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.