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ZFIN ID: ZDB-FIG-180104-23
Sandoval et al., 2017 - A metabolic switch controls intestinal differentiation downstream of Adenomatous polyposis coli (APC).. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 4

Knock down of mpc1 or apc leads to altered mitochondrial respiration and pyruvate metabolism.

(A) Mitochondrial respiration was evaluated by measuring oxygen consumption rates (OCR) in 72 hpf embryos. (B) Triglyceride (TG) levels were determined in lysates prepared from 72 hpf embryos using a colorimetric assay. (C,D) Quantitative RT-PCR analysis of enzymes involved in pyruvate metabolism in apc mo and (C) apcmcr (D) embryos. pyruvate dehydrogenase alpha 1a (pdha1a); pyruvate dehydrogenase kinase, isozyme 1 (pdk1); pyruvate kinase, muscle, a (pkma); citrate synthase (cs). (E) Lactate levels in apc wild type (WT), un-injected apcmcr (UI) or apcmcr embryos injected with human MPC1 mRNA (MPC1 RNA). For figures A–E, values represent mean ± SD. Graph shown above is representative of at least three independent experiments. Statistical significance was analyzed using unpaired t-test. (F,G,H) Gross phenotype (F), alcian blue staining (G) and in situ hybridization for fabp2 (H) in pdha1a, pcxb, and pcxb + pdha1a mo. pcxb (pyruvate carboxylase b). See also Figure 4—figure supplements 1, 2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cs TU + MO3-apc standard conditions Protruding-mouth whole organism RTPCR
fabp2 TU standard conditions Protruding-mouth gut ISH
TU + MO1-pcxb standard conditions Protruding-mouth exocrine pancreas ISH
TU + MO1-pcxb + MO1-pdha1a standard conditions Protruding-mouth exocrine pancreas ISH
TU + MO1-pdha1a standard conditions Protruding-mouth exocrine pancreas ISH
pdha1a TU + MO3-apc standard conditions Protruding-mouth whole organism RTPCR
apchu745/hu745 standard conditions Protruding-mouth whole organism RTPCR
pdk1 TU + MO3-apc standard conditions Protruding-mouth whole organism RTPCR
apchu745/hu745 standard conditions Protruding-mouth whole organism RTPCR
pkma TU + MO3-apc standard conditions Protruding-mouth whole organism RTPCR
apchu745/hu745 standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-mpc1 standard conditions Protruding-mouth aerobic respiration decreased rate, abnormal
Protruding-mouth whole organism triglyceride decreased amount, abnormal
TU + MO1-pcxb standard conditions Long-pec pericardium edematous, abnormal
Protruding-mouth digestive tract development process quality, abnormal
Protruding-mouth exocrine pancreas fabp2 expression decreased amount, abnormal
Protruding-mouth pericardium edematous, abnormal
Day 4 mandibular arch skeleton cartilage development decreased occurrence, abnormal
Day 4 pectoral fin absent, abnormal
TU + MO1-pcxb + MO1-pdha1a standard conditions Long-pec hindbrain increased size, abnormal
Long-pec pericardium edematous, abnormal
Protruding-mouth digestive tract development process quality, abnormal
Protruding-mouth exocrine pancreas fabp2 expression decreased amount, abnormal
Protruding-mouth pericardium edematous, abnormal
Day 4 mandibular arch skeleton cartilage development decreased occurrence, abnormal
Day 4 pectoral fin absent, abnormal
TU + MO1-pdha1a standard conditions Long-pec pericardium edematous, abnormal
Protruding-mouth digestive tract development process quality, abnormal
Protruding-mouth exocrine pancreas fabp2 expression decreased amount, abnormal
Protruding-mouth pericardium edematous, abnormal
Day 4 mandibular arch skeleton cartilage development decreased occurrence, abnormal
Day 4 pectoral fin absent, abnormal
TU + MO3-apc standard conditions Protruding-mouth aerobic respiration decreased rate, abnormal
Protruding-mouth whole organism pdha1a expression decreased amount, abnormal
Protruding-mouth whole organism cs expression decreased amount, abnormal
Protruding-mouth whole organism pdk1 expression decreased amount, abnormal
Protruding-mouth whole organism pkma expression increased amount, abnormal
Protruding-mouth whole organism triglyceride decreased amount, abnormal
apchu745/hu745 standard conditions Protruding-mouth aerobic respiration decreased rate, abnormal
apchu745/hu745 standard conditions Protruding-mouth whole organism pdk1 expression decreased amount, abnormal
apchu745/hu745 standard conditions Protruding-mouth whole organism pdha1a expression decreased amount, abnormal
apchu745/hu745 standard conditions Protruding-mouth whole organism pkma expression increased amount, abnormal
apchu745/hu745 standard conditions Protruding-mouth whole organism lactate increased amount, abnormal
apchu745/hu745 standard conditions Protruding-mouth whole organism triglyceride decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife