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ZFIN ID: ZDB-FIG-180104-18
Sandoval et al., 2017 - A metabolic switch controls intestinal differentiation downstream of Adenomatous polyposis coli (APC).. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 1

mpc1 and mpc2 are downregulated in apcmcr and apc morphant embryos.

(A,B) Quantitative RT-PCR analysis of mpc1 and mpc2 gene expression in apcmcr (A) and apc mo (B) embryos. Values represent mean ± SD. Graph shown above is representative of at least three independent experiments. Statistical significance was analyzed using unpaired t-test. (C,D) Whole mount in situ hybridization for mpc1 and mpc2 in 72 hpf apcmcr (C) and apc mo (D) embryos. (E) Whole mount in situ hybridization for mpc1 and mpc2 in wild type (WT) embryos. head (h), eyes (e), somite (som), vasculature (vas), gut (g), liver (l). (F) Cross sections from 96 hpf WT embryos probed with either mpc1 or mpc2 confirmed gut-specific expression of both genes. See also Figure 1—figure supplement 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mpc1 TU standard conditions Prim-5 eye ISH
Prim-5 head ISH
Prim-5 vasculature ISH
Long-pec somite ISH
Protruding-mouth gut ISH
Protruding-mouth liver ISH
Protruding-mouth pectoral fin ISH
Day 4 gut ISH
TU + MO3-apc standard conditions Protruding-mouth gut ISH
Protruding-mouth head ISH
Protruding-mouth whole organism RTPCR
apchu745/hu745 standard conditions Protruding-mouth gut ISH
Protruding-mouth head ISH
Protruding-mouth whole organism RTPCR
mpc2 TU standard conditions Prim-5 eye ISH
Prim-5 head ISH
Prim-5 somite ISH
Prim-5 vasculature ISH
Long-pec vasculature ISH
Protruding-mouth gut ISH
Protruding-mouth liver ISH
Protruding-mouth pectoral fin ISH
Day 4 gut ISH
TU + MO3-apc standard conditions Protruding-mouth trunk ISH
Protruding-mouth whole organism RTPCR
apchu745/hu745 standard conditions Protruding-mouth trunk ISH
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO3-apc standard conditions Protruding-mouth gut mpc1 expression decreased amount, abnormal
Protruding-mouth head mpc1 expression decreased amount, abnormal
Protruding-mouth trunk mpc2 expression decreased amount, abnormal
Protruding-mouth whole organism mpc2 expression decreased amount, abnormal
Protruding-mouth whole organism mpc1 expression decreased amount, abnormal
apchu745/hu745 standard conditions Protruding-mouth gut mpc1 expression decreased amount, abnormal
Protruding-mouth head mpc1 expression decreased amount, abnormal
Protruding-mouth trunk mpc2 expression decreased amount, abnormal
Protruding-mouth whole organism mpc1 expression decreased amount, abnormal
Protruding-mouth whole organism mpc2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife