header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180104-22
Sandoval et al., 2017 - A metabolic switch controls intestinal differentiation downstream of Adenomatous polyposis coli (APC).. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 3

MPC1 rescues gut phenotype of apc mo and apcmcr.

(A,B) In situ hybridization for fabp2, in 72 hpf WT embryos injected with cont mo, apc mo or both apc mo and human MPC1 RNA (apc mo + MPC1 RNA) (A). In situ hybridization for fabp2 in 72 hpf apc WT, apcmcr or apcmcr injected with human MPC1 mRNA (apcmcr + MPC1 RNA) (B). (C,D) Quantification of injected embryos with differentiated gut as determined by fabp2 staining. Statistical significance was analyzed using Fisher’s exact test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fabp2 TU standard conditions Protruding-mouth gut ISH
TU + MO3-apc standard conditions Protruding-mouth gut ISH
WT standard conditions Protruding-mouth gut ISH
apchu745/hu745 standard conditions Protruding-mouth gut ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO3-apc standard conditions Protruding-mouth gut fabp2 expression decreased amount, abnormal
apchu745/hu745 standard conditions Protruding-mouth gut fabp2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife