ZFIN ID: ZDB-FIG-171106-31
Leong et al., 2015 - Methyl-CpG Binding Protein 2 (Mecp2) Regulates Sensory Function Through Sema5b and Robo2. Frontiers in Cellular Neuroscience   9:481 Full text @ Front. Cell. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5

FIGURE 5. Sema5b and Robo2 rescue Mecp2-induced defects in axonal projections in TG. Representative gel images showing sema3f, sema5b and robo2 mRNA levels regulated by Mecp2 (A). Graph presenting quantitative RT-PCR of the three above mentioned genes in WT or Mecp2 MO injected embryos (B). Graph showing expression of sema3f, sema5b and robo2 mRNA in CTR and mecp2Q63/Q63 mutants using RT-PCR (C). Data are presented as fold expression of each gene normalized with housekeeping gene, beta-actin to value 1. (P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, two-way ANOVA with Bonferroni’s post hoc test). Sema5b and Robo2 rescue axonal projection of TG cells. Representative images showing peripheral projections of trigeminal sensory neurons in embryos injected with mecp2 MO together with mRNA of Sema5b, Robo2 or both (D). Quantitative analysis of total peripheral projections from trigeminal sensory neurons, with graph presenting total length of peripheral projections from trigeminal ganglia per zebrafish (E) (Blue – DAPI, Green – acetylated tubulin, Red – islet1). N > 10 in each group, Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
robo2 AB standard conditions Prim-5 whole organism RTPCR
AB + MO5-mecp2 standard conditions Prim-5 whole organism RTPCR
mecp2fh232/fh232 standard conditions Prim-5 whole organism RTPCR
sema3fa AB standard conditions Prim-5 whole organism RTPCR
AB + MO5-mecp2 standard conditions Prim-5 whole organism RTPCR
mecp2fh232/fh232 standard conditions Prim-5 whole organism RTPCR
sema5ba AB standard conditions Prim-5 whole organism RTPCR
AB + MO5-mecp2 standard conditions Prim-5 whole organism RTPCR
mecp2fh232/fh232 standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO5-mecp2 standard conditions Prim-5 whole organism robo2 expression decreased amount, abnormal
Prim-5 whole organism sema5ba expression decreased amount, abnormal
Prim-5 whole organism sema3fa expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Cellular Neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Cell. Neurosci.