ZFIN ID: ZDB-FIG-171106-32
Leong et al., 2015 - Methyl-CpG Binding Protein 2 (Mecp2) Regulates Sensory Function Through Sema5b and Robo2. Frontiers in Cellular Neuroscience   9:481 Full text @ Front. Cell. Neurosci.
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7

FIGURE 7. Mecp2-mediated defects of axon projections leads to diminished sensory response. Representative images showing arrays of muscle fibers in WT control and Mecp2 morphants (KD) embryos immunostained with monoclonal antibody A4.1025 (myosin) (A). In all panels, anterior is to the left. (Blue-DAPI, red-myosin) Scale bar = 50 μm. Graphs showing rates of response upon tactile stimuli (red arrow in C) from the six experimental groups of WT and KD, including WT and KD coinjected with various rescue constructs indicated on the graph, upon tactile stimuli applied to the same region specified (B,C). Proposed model shows Mecp2 plays a part in primary sensory projections through regulating transcription of Sema5b and Robo2 (C). Each individual dot (B) represents individual embryos. N > 30. (∗∗∗P < 0.0001, one-way ANOVA with Bonferroni’ post hoc test).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO5-mecp2 standard conditions Long-pec muscle striated, normal
Long-pec sensory perception of touch disrupted, abnormal
ZFIN wishes to thank the journal Frontiers in Cellular Neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Cell. Neurosci.