ZFIN ID: ZDB-FIG-171106-29
Leong et al., 2015 - Methyl-CpG Binding Protein 2 (Mecp2) Regulates Sensory Function Through Sema5b and Robo2. Frontiers in Cellular Neuroscience   9:481 Full text @ Front. Cell. Neurosci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

FIGURE 2. Knockdown of Mecp2 affects the axonal projection of trigeminal ganglion (TG) cells. Structure of peripheral dendritic projection and nerve pathways revealed by axon marker, acetylated tubulin (green), a general nucleus marker, DAPI (blue) and a neuronal marker, islet1 (red) at 24 hpf (A). Representative images showing peripheral axonal projections from sensory neurons of TG in wild-type controls (WT, top panel), Mecp2 morphants (second panel from top), Mecp2 morphants rescued by coinjecting mRNA of Mecp2 (third panel from top) and Mecp2 morphants coinjected with p53 MO (lower panel) (A). Quantitative analysis of total peripheral projections of trigeminal sensory neurons, with graph presenting total length of peripheral projections from trigeminal ganglia per zebrafish (B) N > 10. Representative images showing peripheral axonal projections from sensory neurons of TG in wild-type controls (WT, top panel) and mecp2Q63/Q63 mutants (Mecp2-Q63, bottom panel) (C). Graph showing quantitative analysis of peripheral projections (total length) from trigeminal ganglia per zebrafish in wild-type controls and mecp2Q63/Q63 mutants (D). Graph showing total neurite length of controls (CTR) and mecp2Q63/Q63 mutants (Q63) injected with either 500 or 800 μM of mecp2 MO (E). Scale bar = 50 μm. Each individual dot on graph (E) represents individual embryos. N = 30 in each group. (P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.0001, student t-test for (D) and one-way ANOVA with Bonferroni’s post hoc test for (B,E)].

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO4-tp53 + MO5-mecp2 standard conditions Prim-5 trigeminal ganglion axon branchiness, abnormal
Prim-5 trigeminal ganglion axon arborization disrupted, abnormal
AB + MO5-mecp2 standard conditions Prim-5 trigeminal ganglion axon branchiness, abnormal
Prim-5 trigeminal ganglion axon decreased length, abnormal
Prim-5 trigeminal ganglion axon arborization disrupted, abnormal
mecp2fh232/fh232 standard conditions Prim-5 trigeminal ganglion axon decreased length, abnormal
Prim-5 trigeminal ganglion axon arborization disrupted, abnormal
mecp2fh232/fh232 + MO5-mecp2 standard conditions Prim-5 trigeminal ganglion axon decreased length, abnormal
Prim-5 trigeminal ganglion axon arborization disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Cellular Neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Cell. Neurosci.