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ZFIN ID: ZDB-FIG-170209-31
Barske et al., 2016 - Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face. PLoS Genetics   12:e1005967 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Day 5

Fig. 9

Simultaneous loss of prrx1a/b and jag1b further improves ventral cartilages in edn1 mutants.

(A-E) Ventral views of dissected facial skeletons. edn1 mutants have much reduced ventral cartilage in the first (black closed arrowhead) and second (black open arrowhead) arches. Whereas jag1b; edn1 and prrx1a; prrx1b; edn1 mutants show some restoration of ventral cartilage, primarily in the second arch, quadruple prrx1a; prrx1b; jag1b; edn1 mutants show a prominent rescue of second arch Ch cartilage (black open arrowhead) and increased length of first arch M cartilage (black closed arrowhead). (F) Quantification of skeletal rescue in prrx1a; prrx1b; edn1 mutants (left column) and jag1b; edn1 mutants with decreasing numbers of wild-type prrx1a/b alleles (e.g. 4 wild-type alleles = pure jag1b; edn1 mutants; 0 wild-type alleles = quadruple mutant). Examples of no, mild, moderate, or strong rescue of the ceratohyal cartilage are shown in (B-E). Numbers in each genotype, from left to right: 11, 13, 17, 15, 6. (G-J) edn1 mutants show a loss of ventral barx1 expression (magenta, white open arrowhead) and a partial reduction of dlx5a (green), which are partially restored in prrx1a; prrx1b; edn1 triple mutants. The magenta channel is shown by itself in (G'-J'). Scale bar in E = 100 μm; scale bar in J' = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barx1 WT control Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region ISH
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region ISH
prrx1ael558/el558 ; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch ventral region ISH
dlx5a WT control Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region ISH
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region ISH
prrx1ael558/el558 ; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region dlx5a expression decreased amount, abnormal
Prim-25 pharyngeal arch ventral region barx1 expression decreased amount, abnormal
Day 5 pharyngeal arch 1 ventral region decreased amount, abnormal
Day 5 pharyngeal arch 1 ventral region decreased size, abnormal
Day 5 pharyngeal arch 2 ventral region decreased amount, abnormal
Day 5 pharyngeal arch 2 ventral region decreased size, abnormal
Day 5 pharyngeal arch cartilage ventral region decreased amount, abnormal
Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Day 5 pharyngeal arch 1 ventral region morphology, abnormal
Day 5 pharyngeal arch 2 ventral region morphology, abnormal
Day 5 pharyngeal arch 2 ventral region size, ameliorated
Day 5 pharyngeal arch cartilage ventral region amount, ameliorated
Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region dlx5a expression amount, ameliorated
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region barx1 expression amount, ameliorated
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Day 5 pharyngeal arch 1 ventral region morphology, abnormal
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Day 5 pharyngeal arch 2 ventral region morphology, abnormal
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Day 5 pharyngeal arch 2 ventral region size, ameliorated
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Day 5 pharyngeal arch cartilage ventral region amount, ameliorated
prrx1ael558/el558 ; el49Tg/el49Tg ; edn1tf216b/tf216b standard conditions Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
prrx1ael558/el558 ; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch ventral region dlx5a expression decreased amount, abnormal
Prim-25 pharyngeal arch ventral region barx1 expression decreased amount, abnormal
prrx1bel491/el491 ; edn1tf216b/tf216b ; jag1bb1105/b1105 ; prrx1ael558/el558 standard conditions Day 5 ceratohyal cartilage size, ameliorated
Day 5 Meckel's cartilage length, ameliorated
Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.