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ZFIN ID: ZDB-FIG-170209-30
Barske et al., 2016 - Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face. PLoS Genetics   12:e1005967 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-25 to Day 5

Fig. 8

Partially overlapping functions of Prrx1a/b and Jagged-Notch signaling in dorsal cartilage development.

(A-F) The expression of prrx1a and prrx1b (magenta) is largely normal in jag1b mutants (B, D) but is upregulated in ventral arch NCCs (white arrows) and reduced in dorsal NCCs upon forced Notch activation in hsp70I:Gal4; UAS:NICD embryos subjected to a 20–24 hpf heat-shock treatment. dlx2a expression (green) marks all arch NCCs. (G-I) edn1 mutants display a loss of ventral barx1 expression (green) and gain of prrx1b (magenta) (white arrowhead) (H). In jag1b; edn1 mutants, there is partial recovery of ventral barx1 expression in the second arch (white open arrowhead), which corresponds to regions where the ectopic expression of prrx1b is restored to control levels. (J, K) In 7/10 prrx1a; prrx1b mutants, jag1b expression is partially reduced in the dorsal second arch (yellow arrow). (L-O) Dissections of facial cartilage and bone derived from the first two arches show additive phenotypes in jag1b; prrx1a; prrx1b triple mutants. Similar to prrx1a; prrx1b double mutants, triple mutants display ectopic cartilage connecting Pq to the otic cartilage (black arrows). However, similar to jag1b single mutants, jag1b; prrx1a; prrx1b triple mutants also display irregularities in the main body of Pq (black arrowheads). Scale bar in K = 20 μm; scale bar in O = 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barx1 edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ISH
Prim-25 pharyngeal arch 2 ventral region ISH
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch ISH
Prim-25 pharyngeal arch 2 ISH
dlx2a WT control Prim-25 pharyngeal arch neural crest cell ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch neural crest cell ISH
kca3Tg; kca4Tg heat shock Prim-25 pharyngeal arch neural crest cell ISH
prrx1ael558/el558 ; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch neural crest cell ISH
jag1b prrx1ael558/el558 ; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch 2 postero-dorsal region ISH
prrx1a WT control Prim-25 pharyngeal arch ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch ISH
kca3Tg; kca4Tg heat shock Prim-25 pharyngeal arch ISH
prrx1b WT control Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ISH
Prim-25 pharyngeal arch 2 ventral region ISH
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch ISH
kca3Tg; kca4Tg heat shock Prim-25 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 2 ventral region barx1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 ventral region prrx1b expression increased amount, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch barx1 expression mislocalised, abnormal
jag1bb1105/b1105 standard conditions Day 5 palatoquadrate cartilage morphology, abnormal
jag1bb1105/b1105 ; prrx1bel491/el491 ; prrx1ael558/el558 standard conditions Day 5 palatoquadrate cartilage fused with inner ear, abnormal
Day 5 palatoquadrate cartilage mislocalised, abnormal
Day 5 palatoquadrate cartilage morphology, abnormal
kca3Tg; kca4Tg heat shock Prim-25 pharyngeal arch prrx1a expression increased amount, abnormal
Prim-25 pharyngeal arch prrx1b expression increased amount, abnormal
prrx1ael558/el558 ; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch 2 postero-dorsal region jag1b expression decreased amount, abnormal
Day 5 palatoquadrate cartilage fused with inner ear, abnormal
Day 5 palatoquadrate cartilage mislocalised, abnormal
Day 5 palatoquadrate cartilage morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.