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ZFIN ID: ZDB-FIG-170209-25
Barske et al., 2016 - Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face. PLoS Genetics   12:e1005967 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 3

Regulation of barx1+ condensations by Edn1 and Notch.

(A) At 36 hpf, the intermediate sox9a domain (green) only partially overlaps with zones of barx1 expression (magenta) at the ventral and dorsal poles of each arch. The oral ectoderm (oe) and first pharyngeal pouch (p1) are shown for reference. (B, C) jag1b (green) and barx1 (magenta) are anti-correlated in dorsal NCCs at 36 and 48 hpf. (D-J) barx1 expression at 36 hpf in the first and second arches of wild-type controls, mutants, and overexpression embryos. Open arrowheads show the loss of ventral barx1 in edn1 mutants (E) and its restoration in 5/6 jag1b; edn1 mutants (J). The blue arrow in E indicates weak upregulation of barx1 in the intermediate domain of edn1 mutants. Upregulation of barx1 in the dorsal first arch (white arrowhead) and dorsal second arch (white arrow) is seen in Edn1-overexpressing embryos (F), jag1b mutants (G), notch2; notch3 mutants (H), and jag1b; edn1 mutants (J). Dotted lines in (I) show the arches of NICD-overexpression embryos in which barx1 is nearly absent. (K, L) Ectopic barx1 persists in jag1b mutants at least until 48 hpf, but no ectopic expression of sox9a is observed. (M, N) Representative barx1 expression patterns and skeletal preparations in embryos treated with the Notch inhibitor DBZ (10 μM) starting at the indicated time points. Earlier exposure to the DBZ inhibitor correlated with stronger ectopic barx1 expression (M) and more severe and penetrant Notch-type skeletal phenotypes (N). Fractions indicate the number of embryos in each treatment that exhibited unambiguous ectopic barx1 expression in the dorsal first arch (arrowheads in M) or showed posterior Pq malformations (arrowheads in N. DBZ treatment also caused systemic effects, including spinal curvature and cardiac edema, which reduced bone mineralization and led to a general reduction in the size of the craniofacial skeleton. Scale bars in B, C, J, L, M = 20 μm; scale bar in N = 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barx1 WT control Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
WT standard conditions Prim-25 pharyngeal arch ISH
Long-pec pharyngeal arch ISH
WT chemical treatment by environment: dibenzoazepine Prim-25 pharyngeal arch ISH
Prim-25 pharyngeal arch 1 ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 2 ISH
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Long-pec pharyngeal arch ISH
kca3Tg; kca4Tg heat shock Prim-25 pharyngeal arch ISH
Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
notch2el515/el515 ; notch3fh332/fh332 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
jag1b WT standard conditions Prim-25 neural crest cell ISH
Long-pec pharyngeal arch ISH
sox9a WT standard conditions Prim-25 pharyngeal arch ISH
jag1bb1105/b1105 standard conditions Long-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: dibenzoazepine Prim-25 pharyngeal arch barx1 expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 barx1 expression increased distribution, abnormal
Day 4 palatoquadrate arch malformed, abnormal
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 2 barx1 expression decreased amount, abnormal
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch barx1 expression increased distribution, abnormal
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 barx1 expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 barx1 expression increased distribution, abnormal
Long-pec pharyngeal arch barx1 expression increased distribution, abnormal
kca3Tg; kca4Tg heat shock Prim-25 pharyngeal arch barx1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 barx1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 barx1 expression decreased amount, abnormal
notch2el515/el515 ; notch3fh332/fh332 standard conditions Prim-25 pharyngeal arch 1 barx1 expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 barx1 expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.