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ZFIN ID: ZDB-FIG-170209-28
Barske et al., 2016 - Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face. PLoS Genetics   12:e1005967 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-25

Fig. 6

prrx1a and prrx1b are repressed by Edn1 and activated by Bmp4 signaling.

(A-F) Two-color fluorescent in situs of 36 hpf wild-type embryos show that, relative to all arch NCCs (dlx2a, green), prrx1a and prrx1b (magenta) are expressed in dorsal arch NCCs and mesenchyme surrounding the ear (white arrow), as well as in a more limited ventral arch domain (white arrowhead). prrx1a and prrx1b are upregulated in ventral arch NCCs (white open arrowhead) of edn1 mutants and nearly lost upon overexpression of Edn1 in hsp70I:Gal4; UAS:Edn1 embryos subjected to a 20–24 hpf heat-shock treatment. (G, H) prrx1a/b and barx1 are expressed complementarily in the arches of wild types. (I, J) Overexpression of Bmp4 in hsp70I:Gal4; UAS:Bmp4 embryos heat-shocked from 20–24 hpf resulted in broad upregulation of prrx1a/b throughout the arches, with barx1 restricted to domains showing lower prrx1a/b expression. (K) prrx1b overlaps only slightly with jag1b expression at the dorsal-posterior tips of the first and second arches (yellow arrows). (L) Schematic depicting the expression patterns of prrx1a/b (magenta), barx1 (green), and jag1b (blue). Scale bar = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barx1 WT standard conditions Prim-25 pharyngeal arch ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch ISH
dlx2a WT standard conditions Prim-25 pharyngeal arch neural crest cell ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch neural crest cell ISH
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch neural crest cell ISH
jag1b WT standard conditions Prim-25 pharyngeal arch ISH
Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
prrx1a WT standard conditions Prim-25 mesenchyme ISH
Prim-25 pharyngeal arch neural crest cell ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch neural crest cell ISH
Prim-25 pharyngeal arch ventral region ISH
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch neural crest cell ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch ISH
prrx1b WT standard conditions Prim-25 mesenchyme ISH
Prim-25 pharyngeal arch neural crest cell ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch neural crest cell ISH
Prim-25 pharyngeal arch ventral region ISH
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch neural crest cell ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch neural crest cell prrx1b expression increased amount, abnormal
Prim-25 pharyngeal arch neural crest cell prrx1a expression increased amount, abnormal
Prim-25 pharyngeal arch ventral region prrx1a expression increased amount, abnormal
Prim-25 pharyngeal arch ventral region prrx1b expression increased amount, abnormal
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch neural crest cell prrx1a expression decreased amount, abnormal
Prim-25 pharyngeal arch neural crest cell prrx1b expression decreased amount, abnormal
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch prrx1a expression increased amount, abnormal
Prim-25 pharyngeal arch prrx1b expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.