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ZIRC
ZFIN ID: ZDB-FIG-160817-46
Naylor et al., 2016 - BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis. Nature communications   7:12197 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5

Knock down of Cyp26a1 anteriorizes the kidney.

Whole-mount double in situ hybridization analysis at 24 hours post fertilisation (h.p.f.) for nephron segment markers slc4a4 (orange), slc12a1 and slc12a3 (purple) in embryos injected with cyp26a1 morpholino or treated with R115866 or DMSO (vehicle control). Embryos are shown as lateral views with anterior to the left. Arrowhead indicates junction between the DE and DL segments. Scale bar, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc4a4a TU control Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
TU chemical treatment by environment: talarozole Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
TU + MO1-cyp26a1 standard conditions Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
slc12a1 TU control Prim-5 pronephric distal early tubule ISH
TU chemical treatment by environment: talarozole Prim-5 pronephric distal early tubule ISH
TU + MO1-cyp26a1 standard conditions Prim-5 pronephric distal early tubule ISH
slc12a3 TU control Prim-5 pronephric distal late tubule ISH
TU chemical treatment by environment: talarozole Prim-5 pronephric distal late tubule ISH
TU + MO1-cyp26a1 standard conditions Prim-5 pronephric distal late tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment by environment: talarozole Prim-5 pronephric distal early tubule slc12a1 expression mislocalised, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric proximal convoluted tubule slc4a4a expression increased distribution, abnormal
Prim-5 pronephric proximal straight tubule slc4a4a expression increased distribution, abnormal
TU + MO1-cyp26a1 standard conditions Prim-5 anterior/posterior pattern specification involved in pronephros development disrupted, abnormal
Prim-5 pronephric distal early tubule slc12a1 expression mislocalised, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric proximal convoluted tubule slc4a4a expression increased distribution, abnormal
Prim-5 pronephric proximal straight tubule slc4a4a expression increased distribution, abnormal
Prim-5 pronephros wholly anterioralized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.