ZFIN ID: ZDB-FIG-160414-14
Langdon et al., 2016 - Split top: A maternal cathepsin B that regulates dorsoventral patterning and morphogenesis. Development (Cambridge, England)   143(6):1016-28 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Bud to 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to 5-9 somites

Fig. 5

Convergent extension in split top embryos. Expression of (A) brachyury, (B) tbx16, (C,D) pax2.1, (D) krox20 and (C,D) myod indicates an expansion of the midline (probably combined with increased midline mesoderm tissue) and reduced extension in split top mutants. Additionally, whereas krox20 marks rhombomeres 3 and 5 in wild-type embryos, in split top mutants a single krox20 stripe is evident, indicating a loss of either rhombomeres 3 or 5, or a delay in rhombomere 5 expression. Black arrows mark the anterior-most wild-type expression domain and red arrows the anterior-most split top expression domain. The dorsal midline tissue is marked by T-bars in wild-type (black or white) and split top (red) mutants. The brackets indicate the distance from the anterior-most point of the embryo to the neural markers. In A,B, anterior is to top; in C,D, anterior is to left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b TL standard conditions 1-4 somites to 5-9 somites presumptive rhombomere 3 ISH
1-4 somites to 5-9 somites presumptive rhombomere 5 ISH
ctsbap24bdth/+(TL) standard conditions 1-4 somites to 5-9 somites hindbrain neural keel ISH
myod1 TL standard conditions Bud segmental plate ISH
1-4 somites to 5-9 somites somite ISH
ctsbap24bdth/+(TL) standard conditions Bud segmental plate ISH
1-4 somites to 5-9 somites somite ISH
pax2a TL standard conditions Bud neural plate ISH
1-4 somites to 5-9 somites neural plate ISH
ctsbap24bdth/+(TL) standard conditions Bud neural plate ISH
1-4 somites to 5-9 somites segmental plate ISH
tbx16 TL standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
Bud tail bud ISH
ctsbap24bdth/+(TL) standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
Bud tail bud ISH
tbxta TL standard conditions Bud notochord ISH
Bud tail bud ISH
ctsbap24bdth/+(TL) standard conditions Bud notochord ISH
Bud tail bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ctsbap24bdth/+(TL) standard conditions Bud adaxial cell tbx16 expression spatial pattern, abnormal
Bud convergent extension increased occurrence, abnormal
Bud neural plate pax2a expression spatial pattern, abnormal
Bud notochord tbxta expression spatial pattern, abnormal
Bud paraxial mesoderm tbx16 expression spatial pattern, abnormal
Bud segmental plate myod1 expression spatial pattern, abnormal
1-4 somites - 5-9 somites convergent extension increased occurrence, abnormal
1-4 somites - 5-9 somites hindbrain neural keel egr2b expression decreased distribution, abnormal
1-4 somites - 5-9 somites segmental plate pax2a expression spatial pattern, abnormal
1-4 somites - 5-9 somites somite myod1 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development