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ZFIN ID: ZDB-FIG-160414-15
Langdon et al., 2016 - Split top: A maternal cathepsin B that regulates dorsoventral patterning and morphogenesis. Development (Cambridge, England)   143(6):1016-28 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: 30%-epiboly to 75%-epiboly

Fig. 6

Microtubules and actin cytoskeleton are disrupted. (A) Wild-type and split top mutants embryos immunostained to label microtubules in the YCL (N=4-5 and N=5 embryos at each stage, respectively). (B) Wild-type and split top mutant embryos stained with Phalloidin conjugated to Alexa Fluor 568 marks actin in the YCL (N=4-5 embryos at each stage). Bright field (BF) images. (C) High-magnification confocal z-projections of Phalloidin-stained embryos and box plot showing the wild-type and mutant EVL cell morphology and number, respectively. P value is shown. Scale bars: 280µm (A), 240µm (B), 70µm (C).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-tuba IHC TL standard conditions 30%-epiboly external yolk syncytial layer microtubule
IHC 30%-epiboly whole organism microtubule
IHC Shield external yolk syncytial layer microtubule
IHC Shield whole organism microtubule
IHC 75%-epiboly external yolk syncytial layer microtubule
IHC 75%-epiboly whole organism microtubule
IHC ctsbap24bdth/+(TL) standard conditions 30%-epiboly external yolk syncytial layer microtubule
IHC 30%-epiboly whole organism microtubule
IHC Shield external yolk syncytial layer microtubule
IHC Shield whole organism microtubule
IHC 75%-epiboly external yolk syncytial layer microtubule
IHC 75%-epiboly whole organism microtubule
Phenotype Details
Fish Conditions Stage Phenotype
ctsbap24bdth/+(TL) standard conditions 30%-epiboly external yolk syncytial layer actin cytoskeleton absent, abnormal
30%-epiboly external yolk syncytial layer actin cytoskeleton decreased amount, abnormal
30%-epiboly external yolk syncytial layer actin cytoskeleton disorganized, abnormal
30%-epiboly external yolk syncytial layer microtubule absent, abnormal
30%-epiboly external yolk syncytial layer microtubule decreased amount, abnormal
30%-epiboly external yolk syncytial layer microtubule disorganized, abnormal
Shield external yolk syncytial layer actin cytoskeleton absent, abnormal
Shield external yolk syncytial layer actin cytoskeleton decreased amount, abnormal
Shield external yolk syncytial layer actin cytoskeleton disorganized, abnormal
Shield external yolk syncytial layer microtubule absent, abnormal
Shield external yolk syncytial layer microtubule decreased amount, abnormal
Shield external yolk syncytial layer microtubule disorganized, abnormal
75%-epiboly EVL cell decreased amount, abnormal
75%-epiboly EVL cell elongated, abnormal
75%-epiboly EVL cell increased size, abnormal
75%-epiboly external yolk syncytial layer actin cytoskeleton absent, abnormal
75%-epiboly external yolk syncytial layer actin cytoskeleton decreased amount, abnormal
75%-epiboly external yolk syncytial layer actin cytoskeleton disorganized, abnormal
75%-epiboly external yolk syncytial layer microtubule absent, abnormal
75%-epiboly external yolk syncytial layer microtubule decreased amount, abnormal
75%-epiboly external yolk syncytial layer microtubule disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development