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ZIRC
ZFIN ID: ZDB-FIG-160224-5
Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling. Development (Cambridge, England)   143(1):147-59 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec

Fig. 5

reck is expressed in the cerebral endothelium where it is required non-cell autonomously for intracerebral vascularization. (A-C) Confocal lateral views of a 48hpf WT Hb. Anterior, left; dorsal, up. (A) Endothelium is green [Tg(fli1a:eGFP)y1]. (B) Fluorescent intensity of reck transcripts detected via RNA in situ HCR. Warmer and cooler colors represent, respectively, signals of higher and lower fluorescent intensity. reck is expressed in cerebral vessels (MtA, CtAs and PHBC) and BAx. (D) Workflow of cell transplantation experiments. Chimeras were analyzed at ~72hpf. (E-G) Confocal lateral views of the Hb of two chimeras. Anterior, left; dorsal, up. Donor endothelium, green [Tg(kdrl:EGFP)1a116]; host endothelium, red [Tg(kdrl:RFP)s896]; lineage tracer, blue (Rhodamine 647 Dextran). (E,F) Chimera made using WT embryos. Note mosaicism in both the Hb environment (blue) and the CtAs (green and red). (G) Chimera made using a WT donor and a reck y72 host. Note mosaic CtAs (asterisks) containing both WT (green) and mutant (red) endothelial cells. Scale bars: 100┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Long-pec central artery IHC
Long-pec endothelial cell IHC
Long-pec metencephalic artery IHC
Long-pec pharyngeal arch 3-7 IHC
Long-pec primordial hindbrain channel IHC
reck y1Tg standard conditions Long-pec blood vessel endothelial cell ISH
Long-pec central artery ISH
Long-pec metencephalic artery ISH
Long-pec pharyngeal arch 3-7 ISH
Long-pec primordial hindbrain channel ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development