header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-160224-6
Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling. Development (Cambridge, England)   143(1):147-59 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 6

The cerebrovascular expression of the transgenic reporter of canonical Wnt signaling Tg(7xTCF-Xla.Siam:GFP)ia4 is specifically lost in reck y72 mutant embryos. (A-N′′) Confocal images (anterior, left) of 36 and 48hpf Hbs from WT and reck y72. Views: lateral (A-H′′; dorsal, up); dorsal (I-N′′; right side, up). Endothelium, red [Tg(kdrl:RFP)s896]; Wnt reporter, green [Tg(7xTCF-Xla.Siam:GFP)ia4]. In the WT, Wnt reporter expression highlights the PHBCs (A-D′′), CtAs (A-D′′,I-K′′) and additional non-vascular tissues. However, in reck y72 mutants, expression of the Wnt reporter is undetectable in PHBCs (E-H′′,L-N′′) and occasional CtAs (N-N′′), but is present elsewhere. Detail zooms: Regions in white dashed boxes (A,E,I,L) shown in C-C′′,G-G′′,K-K′′ and N-N′′, respectively; regions in yellow dashed boxes (C,G) shown in D-D′′ and H-H′′, respectively. See also Fig. 8.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP ia4Tg; s896Tg standard conditions Prim-25 blood vessel endothelial cell IFL
Long-pec central artery IFL
recky72/y72 ; s896Tg ; ia4Tg standard conditions Prim-25 Not Detected primordial hindbrain channel IFL
Long-pec Not Detected central artery IFL
mCherry ia4Tg; s896Tg standard conditions Prim-25 blood vessel endothelial cell IFL
Prim-25 primordial hindbrain channel IFL
Long-pec blood vessel endothelial cell IFL
Long-pec central artery IFL
recky72/y72 ; s896Tg ; ia4Tg standard conditions Prim-25 blood vessel endothelial cell IFL
Prim-25 primordial hindbrain channel IFL
Long-pec blood vessel endothelial cell IFL
Long-pec central artery IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
recky72/y72 ; s896Tg ; ia4Tg standard conditions Prim-25 primordial hindbrain channel GFP expression absent, abnormal
recky72/y72 ; s896Tg ; ia4Tg standard conditions Prim-25 primordial hindbrain channel canonical Wnt signaling pathway decreased process quality, abnormal
recky72/y72 ; s896Tg ; ia4Tg standard conditions Long-pec brain vasculature lacks parts or has fewer parts of type central artery, abnormal
recky72/y72 ; s896Tg ; ia4Tg standard conditions Long-pec central artery GFP expression absent, abnormal
recky72/y72 ; s896Tg ; ia4Tg standard conditions Long-pec central artery canonical Wnt signaling pathway decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development