header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-160224-4
Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling. Development (Cambridge, England)   143(1):147-59 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4

CtA deficit in reck y72 mutant embryos is due to impaired endothelial cell migration from the perineural PHBCs. (A-E) WT Hb vasculature anatomy (A; anterior half detail) and development (B-E; cross-sections ‘cut’ along plane in A. Dorsal, up. PHBCs, red; BA, dark blue; PCS, light blue; avcs (PCS-connected, yellow; BA-connected, orange), CtAs; green. (F-K) Abundance and distribution of Hb endothelial cells and avcs at 36 and 50hpf in WT and reck y72 embryos. (F-I) Confocal images (50hpf). Endothelium, red [Tg(kdrl:RFP)s896]; endothelial nuclei, green [Tg(kdrl:eGFP-NLS)zf109]. Anterior, left. Scale bars: 100µm. (F,G) Lateral views; dorsal, up. (H,I) Dorsal views (ventral level) of extracerebral vessels; left side, bottom. Arrowheads, avcs (PCS-connected, yellow; BA-connected, orange). (J,K) Quantification and distribution of endothelial cells (J) and abundance of avcs (K). Asterisks and bars are color matched. Asterisks indicate significant differences (P<0.001) between age-matched genotypes (Student′s t-test). n=10 embryos per genotype and stage. Error bars indicate s.d. (L,M) Diagrams of the Hb vasculature phenotypes (anterior half detail) in WT (L) and reck y72 embryos (M). The mutant shows a dramatic CtA deficit, hyperplastic PHBCs and too many avcs. See also Figs S4, S5, Movies 15, 16 and Table S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s896Tg ; recky72/y72 ; ubs1Tg standard conditions Long-pec basilar artery IFL
Long-pec blood vessel endothelial cell nucleus IFL
Long-pec posterior communicating artery IFL
Long-pec primordial hindbrain channel IFL
s896Tg; ubs1Tg standard conditions Long-pec basilar artery IFL
Long-pec blood vessel endothelial cell nucleus IFL
Long-pec central artery IFL
Long-pec posterior communicating artery IFL
Long-pec primordial hindbrain channel IFL
mCherry s896Tg ; recky72/y72 ; ubs1Tg standard conditions Long-pec basilar artery IFL
Long-pec posterior communicating artery IFL
Long-pec primordial hindbrain channel IFL
s896Tg; ubs1Tg standard conditions Long-pec basilar artery IFL
Long-pec central artery IFL
Long-pec posterior communicating artery IFL
Long-pec primordial hindbrain channel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s896Tg ; recky72/y72 ; ubs1Tg standard conditions Long-pec brain vasculature has extra parts of type atrioventricular canal, abnormal
Long-pec brain vasculature lacks all parts of type central artery, abnormal
Long-pec primordial hindbrain channel has extra parts of type blood vessel endothelial cell, abnormal
Long-pec primordial hindbrain channel cell migration involved in sprouting angiogenesis decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development