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ZIRC
ZFIN ID: ZDB-FIG-160224-1
Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling. Development (Cambridge, England)   143(1):147-59 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 1

nft y72 mutant embryos lack intracerebral blood vessels and DRG but have normal body morphology. Confocal (A-H) and bright-field (I-L) lateral images. Anterior, left; dorsal, up. A,B,E,G: 72 hpf; C,D: 48 hpf; F,H: 96 hpf; I-L: 60 hpf. (A,B) Central Arteries (CtAs) are found in WT (A) (white arrowheads) but are missing in nft y72 mutants (B); the other head vessels are present in nft y72. Blood vessels [Tg(kdrl:RFP)s896], red. WT (C) and nft y72 mutants (D) show identical trunk vascular patterns. Endothelium [Tg(fli1a:eGFP)y1], green; somite boundaries, blue (Zygmunt et al., 2011). DRG (yellow circles; red HuC immunofluorescence) are present in WT (E) but absent in nft y72 mutants (G). (F,H) Blood vessels are green [Tg(fli1a:eGFP)y1] and red [Tg(kdrl:RFP)s896]; lymphatics (asterisks) are green only [Tg(fli1a:eGFP)y1]. (J,L) Close-ups of head in I,K. MtA, metencephalic artery; PHBC, primordial hindbrain channel; DLAV, dorsal longitudinal anastomotic vessel; Se, intersegmental vessel; PAC, parachordal chain; Fb, forebrain; Mb, midbrain; Hb, hindbrain. Scale bars: 100µm in A,B, 50µm in C-E,G, 25µm in F,H and 200µm in I-L. See also Fig. S1, Movies 1-14 and Table S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP recky72/y72 ; y1Tg ; s896Tg standard conditions Long-pec blood vessel endothelial cell IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec dorsal root ganglion IFL
Long-pec lymphangioblast cord lymph vessel endothelium IFL
Day 4 thoracic duct lymph vessel endothelium IFL
s896Tg; y1Tg standard conditions Long-pec blood vessel endothelial cell IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec dorsal root ganglion IFL
Long-pec lymphangioblast cord lymph vessel endothelium IFL
Day 4 thoracic duct lymph vessel endothelium IFL
mCherry recky72/y72 ; y1Tg ; s896Tg standard conditions Protruding-mouth metencephalic artery IFL
Protruding-mouth primordial hindbrain channel IFL
Day 4 dorsal aorta IFL
Day 4 posterior cardinal vein IFL
s896Tg; y1Tg standard conditions Protruding-mouth central artery IFL
Protruding-mouth metencephalic artery IFL
Protruding-mouth primordial hindbrain channel IFL
Day 4 dorsal aorta IFL
Day 4 posterior cardinal vein IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT standard conditions Protruding-mouth dorsal root ganglion
IHC Protruding-mouth spinal cord
IHC recky72/y72 standard conditions Protruding-mouth spinal cord
Phenotype Details
Fish Conditions Stage Phenotype
recky72/y72 standard conditions Protruding-mouth dorsal root ganglion morphogenesis decreased process quality, abnormal
Protruding-mouth peripheral nervous system lacks all parts of type dorsal root ganglion, abnormal
recky72/y72 ; y1Tg ; s896Tg standard conditions Long-pec trunk vasculature morphology, normal
Pec-fin head morphology, normal
Protruding-mouth brain vasculature lacks all parts of type central artery, abnormal
Protruding-mouth central artery vasculature development decreased process quality, abnormal
Protruding-mouth heart contraction process quality, normal
Day 4 thoracic duct lymph vessel development process quality, normal
Day 4 trunk vasculature morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development