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ZFIN ID: ZDB-FIG-160224-7
Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling. Development (Cambridge, England)   143(1):147-59 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7

reck y72 mutant embryos show aberrant cerebrovascular expression of the Wnt-responsive markers of barriergenic differentiation Glut1 and plvap. (A-G′′) Confocal lateral images of the 72hpf Hb vasculatures of WT (A-D′′) and reck y72 embryos (E-G′′). Anterior, left; dorsal, up. Endothelium, red [Tg(kdrl:RFP)s896 ]; Glut1 immunofluorescence, green. Colored dashed boxes (A,E) demarcate a region of the following vessels: white, MtA (zooms: B-B′′,F-F′′); yellow, CtAs (zooms: C-C′′); blue, PHBCs (zooms: D-D′′,G-G′′). Merged images of zooms are shown in B′′,C′′,D′′,F′′,G′′. Glut1 decorates the MtA, CtAs and PHBCs of the WT (n=7 embryos). By contrast, Glut1 decorates the MtA, but not the PHBCs, of reck y72 (n=10 embryos). (H,I) Transmitted light images of the 48hpf heads of embryos subjected to whole-mount RNA in situ hybridization with plvap riboprobes. (H) In the WT, plvap is expressed in the dorsal aspect of CtAs but not in the PHBCs (n=57 embryos). (I) In reck y72 mutants, plvap is ectopically expressed in the PHBCs (n=8 embryos). Scale bars: 100µm. Merged images of detail zooms are shown in C′′,D′′,G′′,H′′,K′′,N′′. Scale bars: 100µm. See also Figs S7-S9.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mCherry recky72/y72 ; s896Tg standard conditions Protruding-mouth blood vessel endothelial cell IHC
Protruding-mouth metencephalic artery IHC
Protruding-mouth primordial hindbrain channel IHC
s896Tg standard conditions Protruding-mouth blood vessel endothelial cell IHC
Protruding-mouth central artery IHC
Protruding-mouth metencephalic artery IHC
Protruding-mouth primordial hindbrain channel IHC
plvapb WT standard conditions Long-pec central artery dorsal region ISH
Long-pec Not Detected primordial hindbrain channel ISH
recky72/y72 standard conditions Long-pec primordial hindbrain channel ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-slc2a1 IHC recky72/y72 ; s896Tg standard conditions Protruding-mouth metencephalic artery
IHC Protruding-mouth Not Detected primordial hindbrain channel
IHC s896Tg standard conditions Protruding-mouth blood vessel endothelial cell
IHC Protruding-mouth central artery
IHC Protruding-mouth metencephalic artery
IHC Protruding-mouth primordial hindbrain channel
Phenotype Details
Fish Conditions Stage Phenotype
recky72/y72 standard conditions Long-pec primordial hindbrain channel plvapb expression mislocalised, abnormal
Long-pec primordial hindbrain channel establishment of endothelial blood-brain barrier decreased process quality, abnormal
recky72/y72 ; s896Tg standard conditions Protruding-mouth primordial hindbrain channel ab2-slc2a1 labeling absent, abnormal
Protruding-mouth primordial hindbrain channel establishment of endothelial blood-brain barrier decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development