ZFIN ID: ZDB-FIG-151203-3
Kagermeier-Schenk et al., 2011 - Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization. Developmental Cell   21(6):1129-43 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 50%-epiboly to Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 50%-epiboly to Prim-5

Fig. 3

waif1a Inhibits wnt8 Function in Mesoderm and Neuroectoderm Patterning

(A) Reduction of the forebrain marker otx2 and expansion of the posterior neural marker hoxb1b at the 100% epiboly stage in embryos injected with 10 ng waif1a MO1 or MO2 (23 of 28 embryos MO1, 21 of 24 MO2).

(B) Changes in size of the telencephalic foxg1a expression domain at the one somite stage (bracket) in embryos injected with 2 ng each of wnt8.1 and wnt8.2 MOs (20 of 21 embryos), 160 pg waif1a RNA (36 of 36), 0.4 pg wnt8 RNA (22 of 26), and 4 ng of waif1a MO1 (16 of 25) without or with 2 pg axin1 RNA (17 of 23).

(C) Changes in the size of the dorsal gsc expression domain at the 50% epiboly stage (arrowheads) in embryos injected with 2 ng each of wnt8.1 and wnt8.2 MOs (41 of 42 embryos), 150 pg waif1a RNA (35 of 44), and 10 ng waif1a MO1 (20 of 21 embryos), relative to embryos injected with equimolar amounts of luciferase control RNA plus control MO and in hsp70l:Wnt8-GFP transgenic embryos heat shocked at 30% epiboly (46 of 46 embryos).

(D) Head regions with eyes (arrows) and midbrain-hindbrain boundary (arrowheads) indicated at 24 hpf of embryos injected with 10 ng waif1a MO1 and 0.2 ng standard control MO, or 0.2 ng tcf3a MO plus 10 ng control MO.

(E) Quantification of the tcf3a and waif1a knockdown synergy. Embryos were classified using the criteria introduced in Figure 2A, p < 0.001, chi-square test.

See also Figure S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxg1a WT control 1-4 somites presumptive telencephalon ISH
WT + MO1-tpbga standard conditions 1-4 somites presumptive telencephalon ISH
WT + MO1-wnt8a + MO2-wnt8a standard conditions 1-4 somites presumptive telencephalon ISH
gsc WT control 50%-epiboly whole organism dorsal region ISH
WT + MO1-tpbga standard conditions 50%-epiboly whole organism dorsal region ISH
WT + MO1-wnt8a + MO2-wnt8a standard conditions 50%-epiboly whole organism dorsal region ISH
hoxb1b WT control Bud posterior neural plate ISH
WT + MO1-tpbga standard conditions Bud posterior neural plate ISH
WT + MO2-tpbga standard conditions Bud posterior neural plate ISH
otx2b WT control Bud anterior neural plate ISH
WT + MO1-tpbga standard conditions Bud anterior neural plate ISH
WT + MO2-tpbga standard conditions Bud anterior neural plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tcf7l1a standard conditions Prim-5 eye decreased size, abnormal
WT + MO1-tcf7l1a + MO1-tpbga standard conditions Prim-5 eye absent, abnormal
Prim-5 eye decreased size, abnormal
Prim-5 forebrain decreased size, abnormal
Prim-5 midbrain decreased size, abnormal
Prim-5 notochord aplastic/hypoplastic, abnormal
Prim-5 whole organism wholly dorsalized, abnormal
WT + MO1-tpbga standard conditions 50%-epiboly whole organism dorsal region gsc expression decreased distribution, abnormal
Bud anterior neural plate otx2b expression decreased distribution, abnormal
Bud posterior neural plate hoxb1b expression increased distribution, abnormal
1-4 somites presumptive telencephalon foxg1a expression decreased distribution, abnormal
WT + MO1-wnt8a + MO2-wnt8a standard conditions 50%-epiboly whole organism dorsal region gsc expression increased distribution, abnormal
1-4 somites presumptive telencephalon foxg1a expression increased distribution, abnormal
WT + MO2-tpbga standard conditions Bud anterior neural plate otx2b expression decreased distribution, abnormal
Bud posterior neural plate hoxb1b expression increased distribution, abnormal
Acknowledgments:
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Reprinted from Developmental Cell, 21(6), Kagermeier-Schenk, B., Wehner, D., Ozhan-Kizil, G., Yamamoto, H., Li, J., Kirchner, K., Hoffmann, C., Stern, P., Kikuchi, A., Schambony, A., and Weidinger, G., Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization, 1129-43, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell