ZFIN ID: ZDB-FIG-150311-54
Blasky et al., 2014 - Pard3 regulates contact between neural crest cells and the timing of Schwann cell differentiation but is not essential for neural crest migration or myelination. Developmental dynamics : an official publication of the American Association of Anatomists   243(12):1511-23 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: Day 4

Fig. 6 Schwann cells wrap motor axons but delay mbp expression in the absence of pard3 function. A,B: Representative images of control pard3fh305/+ (A) and MZpard3fh305 mutant (B) embryos, focused on a single motor root. Schwann cells are marked by sox10:memRFP (red) and motor axons are marked by mnx1:GFP (green). Schwann cells ensheath motor axons similarly in both control and mutant. Boxes show orthogonal views of motor nerves at the level of the dashed line. Arrows indicate ensheathed motor axons. C-J: Representative images of mbp RNA expression in wild-type and MZpard3fh305 mutant larvae. Views are from lateral of the trunk with anterior to the left and dorsal up. At 4 dpf, mbp expression is evident at motor nerves (mn, asterisks) (C) and the posterior lateral line nerve (pLLn) (D) of a wild-type larva. By contrast, no motor nerve expression is evident at motor nerves of a MZpard3fh305 mutant larva (E) although pLLn expression appears normal (F). At 4.5 dpf, mbp expression is similar in wild-type and MZpard3fh305 mutant larvae at both motor nerves and the pLLn (G-J). Scale bar = 15 µM in A,B; 100 µM in C-J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP pard3abfh305/+; ml2Tg; vu234Tg(AB) standard conditions Day 4 trunk motor neuron IFL
pard3abfh305/fh305; ml2Tg; vu234Tg standard conditions Day 4 trunk motor neuron IFL
mbpa WT standard conditions Day 4 posterior lateral line nerve ISH
Day 4 trunk nerve ISH
pard3abfh305/fh305 standard conditions Day 4 posterior lateral line nerve ISH
Day 4 Not Detected trunk nerve ISH
mRFP pard3abfh305/+; ml2Tg; vu234Tg(AB) standard conditions Day 4 myelinating Schwann cell IFL
pard3abfh305/fh305; ml2Tg; vu234Tg standard conditions Day 4 myelinating Schwann cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.