ZFIN ID: ZDB-FIG-150311-52
Blasky et al., 2014 - Pard3 regulates contact between neural crest cells and the timing of Schwann cell differentiation but is not essential for neural crest migration or myelination. Developmental dynamics : an official publication of the American Association of Anatomists   243(12):1511-23 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 4 Pard3 mediates contact inhibition between neural crest cells. A-F: Individual neural crest cell tracings represented as colored objects. A,B: Representative images of cranial neural crest cell migration in control (A) and mutant (B) embryos. In some regions, neural crest cells appear more tightly packed in mutant than in control. C,D: Representative images of trunk neural crest cells in control (C) and mutant (D) embryos; lateral view. Neural crest cells form chains elongated in the direction of migration in the control embryo (C). By contrast, neural crest cells appear less elongated and to be less well organized as chains in the mutant (D). E,F: Representative frames captured from time-lapse movies of trunk neural crest cell delamination in control (E) and mutant (F) embryos. Views are from dorsal with anterior to the left. Numbers denote time elapsed, in minutes, from start of imaging. Embryos are siblings and imaged sequentially in the same chamber. Neural crest cells in control embryos (n = 30) retract cell membrane upon contact with neighboring cells. By contrast, neural crest cells in mutant embryos (n=24) fail to retract cell membrane and maintain cell overlap (F). G: Quantification of trunk neural crest cell membrane overlap during delamination (pard3fh305/+, n= 3 embryos, 43 cells; MZpard3fh305, n = 4 embryos, 47 cells; ***P = 0.0001; Student′s t-test). Scale bar = 25 ┬ÁM.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pard3abfh305/fh305 standard conditions 14-19 somites - Prim-5 cranial neural crest cell broadly articulated with cranial neural crest cell, abnormal
14-19 somites - Prim-5 cranial neural crest condensed, abnormal
14-19 somites - Prim-5 trunk neural crest cell broadly articulated with trunk neural crest cell, abnormal
14-19 somites - Prim-5 trunk neural crest cell contact inhibition disrupted, abnormal
14-19 somites - Prim-5 trunk neural crest cell disorganized, abnormal
14-19 somites - Prim-5 trunk neural crest cell shape, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.