ZFIN ID: ZDB-FIG-150311-51
Blasky et al., 2014 - Pard3 regulates contact between neural crest cells and the timing of Schwann cell differentiation but is not essential for neural crest migration or myelination. Developmental dynamics : an official publication of the American Association of Anatomists   243(12):1511-23 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Day 5

Fig. 3 pard3 is not essential for neural crest migration. A,B: Representative images of cranial neural crest marked by Tg(sox10:memRFP) reporter expression in 30 hpf control pard3fh305/+ (A) and MZpard3fh305 mutant (B) embryos. View is from lateral with anterior to the left and dorsal up. Arrows indicate migrating groups of neural crest cells. In some regions, indicated by the outlined boxes and magnified in the insets, neural crest cells appear more tightly packed in mutant than in control. ov, otic vesicle. C,D: Representative images of jaw structures at 5 dpf in wild-type (C) and MZpard3fh305 mutant (B) larvae. The jaw in the mutant larva appears similar to that of wild-type. E,F: Representative frames captured from time-lapse movies of trunk neural crest marked by Tg(sox10:memRFP) reporter expression. Motor neurons and axons are marked by Tg(mnx1:GFP) expression (green) in the final frames of the sequences. Images are from lateral with anterior to the left and dorsal up. Numbers indicate time, in minutes, elapsed from beginning of imaging. Images were captured from 18–24 hpf. In the control embryo (E), neural crest cells form streams (asterisks) with little overlap at points of contact (arrows). By contrast, neural crest cells appear less organized and overlap more in the MZpard3fh305 mutant embryo (F). Scale bar = 100 µM in E,F.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP pard3abfh305/+; ml2Tg; vu234Tg(AB) standard conditions 14-19 somites to Prim-5 trunk motor neuron IFL
pard3abfh305/fh305; ml2Tg; vu234Tg standard conditions 14-19 somites to Prim-5 trunk motor neuron IFL
mRFP pard3abfh305/+; ml2Tg; vu234Tg(AB) standard conditions 14-19 somites to Prim-5 trunk neural crest cell IFL
pard3abfh305/+; vu234Tg standard conditions Prim-15 head neural crest cell IFL
pard3abfh305/fh305; ml2Tg; vu234Tg standard conditions 14-19 somites to Prim-5 trunk neural crest cell IFL
pard3abfh305/fh305; vu234Tg standard conditions Prim-15 head neural crest cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pard3abfh305/fh305 standard conditions Prim-15 neural tube neural crest cell migration temporal distribution quality, normal
Day 5 ventral mandibular arch neural crest cell migration process quality, normal
pard3abfh305/fh305; ml2Tg; vu234Tg standard conditions 14-19 somites - Prim-5 cranial neural crest condensed, abnormal
14-19 somites - Prim-5 trunk neural crest cell disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.