ZFIN ID: ZDB-FIG-140602-14
Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements. Developmental Biology   390:247-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3

prdm12b is required for eng1b expression in the p1 domain. (A–Q) eng1b Expression in 24hpf wild type (A, B), control MO-injected (C–G), PTB MO-injected (H–L), PSB MO-injected (M–Q) embryos. (R–U) eng1b and nkx2.2 expression in 24 hpf wild type (R, S) and PTB MO-injected (T, U) embryos. (V, X) evx1 Expression in wild-type (V) and PTB MO-injected (X) embryos. (W, Y) vsx2 Expression in wild-type (W) and PTB MO-injected (Y) embryos. Embryos are shown in dorsal (A, C, H, M, V–Y) or lateral (B, D, I, N, R, T) view with anterior to the left, or in cross section (E–G, J–L, O–Q, S, U) with dorsal to the top. Sections were taken through r4, the hindbrain, the caudal hindbrain or the level of somites 9–12, as indicated. (Z) Quantification of eng1b phenotype. Scale bars are 100 μm. Brackets and asterisks indicate r4, arrows mark V1 interneurons and arrowheads mark somites. MHB – midbrain–hindbrain boundary, HB – hindbrain, CHB – caudal hindbrain, and SC – spinal cord.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
en1b WT control Prim-5 CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 muscle pioneer ISH
Prim-5 rhombomere 4 ISH
WT + MO1-prdm12b standard conditions Prim-5 Not Detected CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 muscle pioneer ISH
Prim-5 rhombomere 4 ISH
WT + MO2-prdm12b standard conditions Prim-5 Not Detected CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 muscle pioneer ISH
evx1 WT control Prim-5 spinal cord interneuron ISH
WT + MO1-prdm12b standard conditions Prim-5 spinal cord interneuron ISH
nkx2.2a WT control Prim-5 spinal cord interneuron ISH
WT + MO1-prdm12b standard conditions Prim-5 spinal cord interneuron ISH
vsx2 WT control Prim-5 spinal cord interneuron ISH
WT + MO1-prdm12b standard conditions Prim-5 spinal cord interneuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm12b standard conditions Prim-5 spinal cord lacks all parts of type CiA, abnormal
WT + MO1-prdm12b standard conditions Prim-5 ventral spinal cord interneuron specification decreased process quality, abnormal
WT + MO2-prdm12b standard conditions Prim-5 spinal cord lacks all parts of type CiA, abnormal
Prim-5 ventral spinal cord interneuron specification decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.