ZFIN ID: ZDB-FIG-140602-18
Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements. Developmental Biology   390:247-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Day 6
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Day 6

Fig. 7

Loss of prdm12b has a transient effect on the number of OPC cells. (A–R, V–Y) Expression of olig2 (A–F), sox10 (G–L), dm20 (M–R), MBP (V, W) or fluoromyelin (X, Y) in wild type (A, B, G, H, M–O, V, X), control MO-injected (C, I), PTB MO-injected (D, E, J, K, P–R, W, Y) and PSB MO-injected (F, L) embryos. All embryos are in dorsal view with anterior to the left. Scale bars are 100 μm (A–R) or 35 μm (V–Y). Arrowheads indicate unaffected regions of sox10 expression, while arrows indicate affected regions. Red circles demark the otic vesicles. (S–U) Quantification of reduction in olig2 (S), sox10 (T) and dm20 (U) expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
olig2 WT control Prim-25 hindbrain glioblast (sensu Vertebrata) ISH
Long-pec hindbrain glioblast (sensu Vertebrata) ISH
WT + MO1-prdm12b standard conditions Prim-25 hindbrain glioblast (sensu Vertebrata) ISH
Long-pec hindbrain glioblast (sensu Vertebrata) ISH
plp1a WT control Protruding-mouth hindbrain oligodendrocyte ISH
Day 4 hindbrain oligodendrocyte ISH
Day 6 hindbrain oligodendrocyte ISH
WT + MO1-prdm12b standard conditions Protruding-mouth hindbrain oligodendrocyte ISH
Day 4 hindbrain oligodendrocyte ISH
Day 6 hindbrain oligodendrocyte ISH
sox10 WT control Prim-25 hindbrain glioblast (sensu Vertebrata) ISH
Long-pec hindbrain glioblast (sensu Vertebrata) ISH
WT + MO1-prdm12b standard conditions Prim-25 hindbrain glioblast (sensu Vertebrata) ISH
Long-pec hindbrain glioblast (sensu Vertebrata) ISH
vsx2 WT + MO1-prdm12b standard conditions Day 4 hindbrain oligodendrocyte ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mbp IHC WT control Day 4 hindbrain oligodendrocyte
IHC WT + MO1-prdm12b standard conditions Day 4 hindbrain oligodendrocyte
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm12b standard conditions Prim-25 hindbrain has fewer parts of type oligodendrocyte, abnormal
Long-pec hindbrain has fewer parts of type oligodendrocyte, abnormal
Day 4 central nervous system myelination process quality, normal
Day 6 central nervous system myelination process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.