ZFIN ID: ZDB-FIG-140602-16
Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements. Developmental Biology   390:247-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5

prdm12b Defines the dorsal boundary of nkx6.1 expression and regulates the size of the p1 domain. (A–N) Expression of dbx1a/nkx6.1 (A, H), pax3/nkx6.1 (B, I), evx1/nkx2.2 (C, J), pax3/nkx2.2 (D, K), vsx2/nkx2.2 (E, L), irx3/nkx2.2 (F, M) and irx3/olig2 (G, N) in wild-type (A–G) or PTB MO-injected (H–N) 24 hpf embryos. Panels represent cross sections through the hindbrain with dorsal to the top. (O) Quantification of dorsoventral patterning defects using ~100 sections from 16 embryos for each gene pair. (P) Diagram of dorsoventral gene expression in wild type (left half) and prdm12b MO-injected (right half) embryos. (Q) Quantification of the distance between various gene expression domains using 10 representative sections for each gene pair. Arrows indicate ectopic nkx6.1+ cells and brackets indicate unlabeled tissue between markers. Scale bar is 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a WT control Prim-5 hindbrain dorso-medial region ISH
Prim-5 hindbrain ventro-lateral region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain dorso-medial region ISH
Prim-5 hindbrain ventro-lateral region ISH
evx1 WT control Prim-5 hindbrain lateral region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain lateral region ISH
irx3a WT control Prim-5 hindbrain dorso-medial region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain dorso-medial region ISH
nkx2.2a WT control Prim-5 hindbrain ventro-medial region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain ventro-medial region ISH
nkx6.1 WT control Prim-5 hindbrain ventro-medial region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain ventro-medial region ISH
olig2 WT control Prim-5 hindbrain ventral region ISH
Prim-5 hindbrain ventro-medial region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain ventral region ISH
Prim-5 hindbrain ventro-medial region ISH
pax3a WT control Prim-5 hindbrain dorsal region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain dorsal region ISH
vsx2 WT control Prim-5 hindbrain ventro-lateral region ISH
WT + MO1-prdm12b standard conditions Prim-5 hindbrain ventro-lateral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm12b standard conditions Prim-5 hindbrain interneuron neuron fate specification process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.