header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-131213-14
Geng et al., 2013 - Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene. Development (Cambridge, England)   40(21):4362-4374 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4 Expression of extracellular matrix (ECM) genes and other semicircular canal markers is altered in the lauscher mutant ear. (A-JJ) Expression of ECM structural and enzyme genes in lau mutant ears at 76 hpf, 4 dpf and 5 dpf. Expression of the HA binding hapln1a (A-F) and the versican genes vcana and vcanb (M-X) is highly upregulated in mutant canal tissue. Expression of hapln3 is normally upregulated in wild-type ears on canal projection fusion (G,I,K); in the mutant, fusion fails, and transcript levels remain low (H,J,L). Antibody staining for type II Collagen (Y-DD) shows precocious protein accumulation at 76 hpf in mutants (Y,Z), persisting at 4-5 dpf (AA-DD); aberrant canal tissue is visible. Genes coding for enzymes for chondroitin synthesis (chsy1, EE,FF) and HA production (has3, GG,HH; ugdh, II,JJ) are upregulated in mutants at 76 hpf. (KK-PP) The canal markers aldh1a3, bmp7b and sox9b are upregulated in the unfused projections in the lau mutant ear. wt, wild type; sib, phenotypically wild-type sibling. Mutant alleles are shown on the panels. Scale bars: in A, 50 μm (applies to columns 1 and 2); in C, 50 μm (applies to all other panels).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a3 WT standard conditions Protruding-mouth inner ear ISH
Protruding-mouth semicircular canal ISH
adgrg6tk256a/tk256a standard conditions Protruding-mouth inner ear ISH
Protruding-mouth semicircular canal ISH
bmp7b WT standard conditions Day 4 semicircular canal ISH
adgrg6tb233c/tb233c standard conditions Day 4 semicircular canal ISH
chsy1 WT standard conditions Protruding-mouth inner ear ISH
adgrg6tb233c/tb233c standard conditions Protruding-mouth inner ear ISH
hapln1a WT standard conditions Protruding-mouth inner ear ISH
Day 4 inner ear ISH
Day 5 inner ear ISH
adgrg6fr24/fr24 standard conditions Protruding-mouth semicircular canal ISH
adgrg6tb233c/+; adgrg6tk256a/+ standard conditions Day 4 semicircular canal ISH
adgrg6tb233c/tb233c standard conditions Day 5 semicircular canal ISH
hapln3 WT standard conditions Protruding-mouth otic vesicle protrusion ISH
Protruding-mouth semicircular canal ISH
Day 4 semicircular canal ISH
Day 5 semicircular canal ISH
adgrg6tb233c/+; adgrg6tk256a/+ standard conditions Day 4 semicircular canal ISH
adgrg6tb233c/tb233c standard conditions Protruding-mouth otic vesicle protrusion ISH
Protruding-mouth semicircular canal ISH
Day 5 semicircular canal ISH
has3 WT standard conditions Day 4 inner ear ISH
adgrg6tb233c/tb233c standard conditions Day 4 inner ear ISH
sox9b WT standard conditions Protruding-mouth semicircular canal ISH
adgrg6fr24/fr24 standard conditions Protruding-mouth semicircular canal ISH
ugdh WT standard conditions Day 5 inner ear ISH
adgrg6tb233c/tb233c standard conditions Day 5 inner ear ISH
vcana WT standard conditions Protruding-mouth dorsolateral septum ISH
Day 4 dorsolateral septum ISH
Day 5 dorsolateral septum ISH
adgrg6fr24/fr24 standard conditions Protruding-mouth otic vesicle protrusion ISH
Protruding-mouth semicircular canal ISH
Day 5 semicircular canal ISH
adgrg6tb233c/tb233c standard conditions Day 4 semicircular canal ISH
vcanb WT standard conditions Protruding-mouth dorsolateral septum ISH
Day 4 dorsolateral septum ISH
Day 5 dorsolateral septum ISH
adgrg6tb233c/+; adgrg6tk256a/+ standard conditions Day 4 semicircular canal ISH
adgrg6tb233c/tb233c standard conditions Protruding-mouth otic vesicle protrusion ISH
Protruding-mouth semicircular canal ISH
Day 5 semicircular canal ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC WT standard conditions Protruding-mouth inner ear
IHC Day 4 inner ear
IHC Day 5 inner ear
IHC adgrg6tb233c/tb233c standard conditions Protruding-mouth inner ear
IHC Protruding-mouth otic vesicle protrusion
IHC Protruding-mouth semicircular canal
IHC Day 4 inner ear
IHC Day 4 semicircular canal
IHC Day 5 inner ear
IHC Day 5 semicircular canal
Phenotype Details
Fish Conditions Stage Phenotype
adgrg6fr24/fr24 standard conditions Protruding-mouth otic vesicle protrusion disorganized, abnormal
Protruding-mouth semicircular canal disorganized, abnormal
Protruding-mouth semicircular canal fusion arrested, abnormal
Day 5 otic vesicle protrusion disorganized, abnormal
Day 5 semicircular canal disorganized, abnormal
Day 5 semicircular canal fusion arrested, abnormal
adgrg6tb233c/+; adgrg6tk256a/+ standard conditions Day 4 otic vesicle protrusion disorganized, abnormal
Day 4 semicircular canal disorganized, abnormal
Day 4 semicircular canal fusion arrested, abnormal
adgrg6tb233c/tb233c standard conditions Protruding-mouth otic vesicle protrusion disorganized, abnormal
Protruding-mouth semicircular canal disorganized, abnormal
Protruding-mouth semicircular canal fusion arrested, abnormal
Day 4 otic vesicle protrusion disorganized, abnormal
Day 4 semicircular canal disorganized, abnormal
Day 4 semicircular canal fusion arrested, abnormal
Day 5 otic vesicle protrusion disorganized, abnormal
Day 5 semicircular canal disorganized, abnormal
Day 5 semicircular canal fusion arrested, abnormal
adgrg6tk256a/tk256a standard conditions Protruding-mouth otic vesicle protrusion disorganized, abnormal
Protruding-mouth semicircular canal disorganized, abnormal
Protruding-mouth semicircular canal fusion arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development