header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-131105-2
Wada et al., 2013 - Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish. Current biology : CB   23(16):1559-65 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 2

Wnt Signaling Positively Regulates Neuromast Size, Whereas Dkk2/Krm Signaling Negatively Regulates It

(A) Neuromast O2 reaches its final size at 80 hpf in wild-type embryos. By contrast, O2 reaches its final size before 48 hpf, and the number of hair cells does not change over time in dkk2-overexpressing embryos.

(B) Manipulations of Wnt signaling affect hair cell numbers. Mean ± SEM is indicated. p < 0.001 (t test).

(C–E) Knockdown of dkk2 (D) or krm (E) gene function increases neuromast size and hair cell number, as revealed by DiAsp labeling.

(F and G) Wnt inhibition by overexpression of dkk2 reduces neuromast size, number of hair cells (F), and number of dividing cells (G). Scale bar represents 20 μm.

(H) Pharmacological blockade of cell division phenocopies inhibition of Wnt signaling.

See also Figures S1–S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf106Tg standard conditions Long-pec neuromast IHC
zf106Tg + MO2-dkk2 standard conditions Long-pec neuromast IHC
zf106Tg + MO4-kremen1 standard conditions Long-pec neuromast IHC
RFP zf422Tg; zf424Tg standard conditions Prim-25 neuromast IHC
Long-pec neuromast IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dkk1a standard conditions Long-pec neuromast has normal numbers of parts of type neuromast hair cell, normal
WT + MO2-dkk2 standard conditions Long-pec neuromast has extra parts of type neuromast hair cell, abnormal
WT + MO3-lef1 standard conditions Long-pec neuromast has fewer parts of type neuromast hair cell, abnormal
WT + MO4-kremen1 standard conditions Long-pec neuromast has extra parts of type neuromast hair cell, abnormal
WT + MO5-dkk1b standard conditions Long-pec neuromast has normal numbers of parts of type neuromast hair cell, normal
nk73aGt; zf424Tg standard conditions Long-pec neuromast has fewer parts of type neuromast hair cell, abnormal
nk73aGt; zf424Tg + MO4-kremen1 standard conditions Long-pec neuromast has normal numbers of parts of type neuromast hair cell, normal
nk73aGt; zf425Tg standard conditions Long-pec neuromast has extra parts of type neuromast hair cell, abnormal
zf106Tg + MO2-dkk2 standard conditions Long-pec neuromast has extra parts of type neuromast hair cell, abnormal
Long-pec neuromast increased area, abnormal
Long-pec neuromast increased size, abnormal
zf106Tg + MO4-kremen1 standard conditions Long-pec neuromast has extra parts of type neuromast hair cell, abnormal
Long-pec neuromast increased area, abnormal
Long-pec neuromast increased size, abnormal
zf422Tg; zf423Tg standard conditions Long-pec neuromast has fewer parts of type neuromast hair cell, abnormal
zf422Tg; zf424Tg standard conditions Prim-25 neuromast mitotic cell cycle decreased rate, abnormal
Long-pec neuromast decreased area, abnormal
Long-pec neuromast decreased size, abnormal
Long-pec neuromast has fewer parts of type neuromast hair cell, abnormal
Long-pec - Day 4 neuromast has fewer parts of type neuromast hair cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.