ZFIN ID: ZDB-FIG-131105-4
Wada et al., 2013 - Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish. Current biology : CB   23(16):1559-65 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 4

Inhibition of Wnt Signaling Prevents Regeneration of Hair Cells

(A–D) Regeneration of hair cells in normal embryos. New hair cells are regenerated within 24 hr after ablation.

(E–H) In dkk2-overexpressing embryos, where Wnt signaling is inhibited, new hair cells never form (G and H). Scale bar in (E) represents 20 μm.

(I) Proposed mechanism for neuromast size control, based on a regulatory network comprising a negative feedback step.

(J) Sequence of events leading to homeostasis in the case of budding of a new neuromast and in the case of hair cell regeneration. Large circles indicate neuromasts; smaller circles indicate hair cells. Wnt signaling (green) is active whenever Dkk (red) is absent. dkk2 mRNA (orange) is expressed in differentiating and mature hair cells. Dkk2 protein (red) secreted by the hair cells reaches the peripheral cells and inhibits Wnt signaling (i.e., cell proliferation). The size of neuromasts is maintained during both budding and regeneration.

See also Figure S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh1a zf106Tg chemical treatment: pharmaceutical Day 4 neuromast hair cell IHC
zf106Tg; zf422Tg; zf424Tg chemical treatment: pharmaceutical Day 4 Not Detected neuromast hair cell IHC
EGFP zf106Tg standard conditions Protruding-mouth neuromast IHC
zf106Tg chemical treatment: pharmaceutical Protruding-mouth neuromast IHC
Day 4 neuromast IHC
zf106Tg; zf422Tg; zf424Tg standard conditions Protruding-mouth neuromast IHC
zf106Tg; zf422Tg; zf424Tg chemical treatment: pharmaceutical Protruding-mouth neuromast IHC
Day 4 neuromast IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf106Tg; zf422Tg; zf424Tg chemical treatment: pharmaceutical Day 4 neuromast hair cell regeneration decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.