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ZFIN ID: ZDB-FIG-131105-3
Wada et al., 2013 - Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish. Current biology : CB   23(16):1559-65 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 3

Wnt Signaling Positively Regulates Neuromast Size, Whereas Dkk2/Krm Signaling Negatively Regulates It

(A) Neuromast O2 reaches its final size at 80 hpf in wild-type embryos. By contrast, O2 reaches its final size before 48 hpf, and the number of hair cells does not change over time in dkk2-overexpressing embryos.

(B) Manipulations of Wnt signaling affect hair cell numbers. Mean ± SEM is indicated. p < 0.001 (t test).

(C–E) Knockdown of dkk2 (D) or krm (E) gene function increases neuromast size and hair cell number, as revealed by DiAsp labeling.

(F and G) Wnt inhibition by overexpression of dkk2 reduces neuromast size, number of hair cells (F), and number of dividing cells (G). Scale bar represents 20 μm.

(H) Pharmacological blockade of cell division phenocopies inhibition of Wnt signaling.

See also Figures S1–S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a WT standard conditions Prim-25 neuromast hair cell ISH
apchu745/hu745 standard conditions Prim-25 neuromast hair cell ISH
zf422Tg; zf423Tg standard conditions Prim-25 neuromast hair cell ISH
dkk2 WT standard conditions Prim-25 neuromast central region ISH
apchu745/hu745 standard conditions Prim-25 neuromast ISH
zf422Tg; zf423Tg standard conditions Prim-25 neuromast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apchu745/hu745 standard conditions Prim-25 neuromast has extra parts of type neuromast hair cell, abnormal
zf422Tg; zf423Tg standard conditions Prim-25 neuromast has fewer parts of type neuromast hair cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.