header logo image header logo text
Downloads Login
General Information
ZFIN ID: ZDB-FIG-131105-1
Wada et al., 2013 - Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish. Current biology : CB   23(16):1559-65 Full text @ Curr. Biol.
Anatomical Terms:
Stage Range: Prim-25 to Day 5
Observed In:
Stage: Prim-25

Fig. 1

Wnt Signaling and Expression of dkk2 Are Associated with Neuromast Proliferation

(A) Schematic drawing of a neuromast in transverse view.

(B) Schematic representation of Wnt signaling through its receptor, Frizzled (Fzd), and of its inhibition by Dickkopf (Dkk) signaling through its receptor, Kremen (Krm). out, extracellular compartment; in, intracellular compartment.

(C) Wnt signaling is detectable only in budding cells (OP2), but not in mature neuromast (OP1), as revealed in a Wnt-responsive GFP reporter line.

(D) The expression of dGFP gradually subsides as hair cells (labeled with atoh:rfp) are formed in O2.

(E) Anti-phosphohistone H3 (pH3) labeling indicates dividing cells in budding neuromast (OP2), but not in mature neuromast (OP1).

(F) Number of pH3-positive cells per neuromast. Mean ± SEM is indicated. p < 0.001, p < 0.01 (t test).

(G and H) dkk2 mRNA is expressed by cells in the center of neuromasts O1 and O2.

(I–L2) Expression profile of dkk2 during neuromast budding. dkk2 mRNA expression coincides with neuromast maturation. The budding structures are outlined.

(M) dkk2 mRNA (blue) is present in hair cells (brown).

(N and O) dkk2 expression is reduced in O2 after ablation of hair cells but is not affected in immature neuromast IO4.

(P) atoh1a mRNA is present in O2 and IO4.

Scale bars represent 20 μm. See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a WT standard conditions Long-pec neuromast hair cell ISH
d2EGFP kyu1Tg standard conditions Protruding-mouth head neuromast IHC
kyu1Tg; nns8Tg standard conditions Day 4 head neuromast IFL
Day 5 head neuromast IFL
dkk2 WT standard conditions Long-pec neuromast hair cell ISH
WT chemical treatment: pharmaceutical Long-pec neuromast hair cell ISH
zf106Tg standard conditions Prim-25 neuromast central region ISH
Protruding-mouth to Day 4 neuromast central region ISH
EGFP zf106Tg standard conditions Prim-25 head neuromast IHC
Protruding-mouth head neuromast IHC
Protruding-mouth to Day 4 head neuromast IHC
Tomato kyu1Tg; nns8Tg standard conditions Day 4 neuromast hair cell IFL
Day 5 neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apchu745/hu745 standard conditions Prim-25 neuromast mitotic cell cycle increased rate, abnormal
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.