ZFIN ID: ZDB-FIG-131017-7
MacDonald et al., 2013 - The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon. Developmental Biology   381(1):276-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 1 The expression domains of the ascl1a, dlx, and gad1b genes overlap in the forebrain of the embryonic zebrafish. Single z sections on triple fluorescent in situ hybridizations. (A) In the forebrain lateral view of a WT embryo, the dlx1a and ascl1a are co-expressed throughout the telencephalon and prethalamus at 24 hpf, while ascl1b is not co-expressed at this stage. (B) The dlx1a, dlx5a, and gad1b genes are co-expressed in the telencephalon, prethalamus, and hypothalamus at 24 hpf. (C) The dlx1a, dlx5a, and gad1b genes continue to be co-expressed in the telencephalon and prethalamus at 48 hpf. The gad1b expression is increased in the dorsal tip of the telencephalon (arrow). The plane of section for (D) is shown as a dotted line in C′′′. (D) Cross-section showing the co-expression of dlx1a, dlx5a, and gad1b in the prethalamus (arrow). The rostral thalamus (arrowhead) is positive for gad1b expression but not dlx1 or dlx5a. Tel=telencephalon, Pth=prethalamus, Hy=hypothalamus, rTh=rostral thalamus. Scale bar in A–D=100 μm and in E=40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1a WT standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
ascl1b WT standard conditions Prim-5 Not Detected telencephalon ISH
Prim-5 Not Detected ventral thalamus ISH
dlx1a WT standard conditions Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
Long-pec telencephalon ISH
Long-pec ventral thalamus ISH
dlx5a WT standard conditions Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
Long-pec telencephalon ISH
Long-pec ventral thalamus ISH
gad1b WT standard conditions Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
Long-pec telencephalon ISH
Long-pec ventral thalamus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 381(1), MacDonald, R.B., Pollack, J.N., Debiais-Thibaud, M., Heude, E., Talbot, J.C., and Ekker, M., The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon, 276-85, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.