ZFIN ID: ZDB-FIG-131017-8
MacDonald et al., 2013 - The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon. Developmental Biology   381(1):276-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Fig. 2 Ascl1a function is required for proper expression of the dlx and gad1b genes in the forebrain at 24 hpf. (A, B) Expression of dlx1a and dlx2a is reduced in the prethalamus and hypothalamus (asterisk), but not affected in the telencephalon of ascl1a morphants compared to control embryos. (C) The expression of dlx5a is particularly reduced in the prethalamus and hypothalamus in ascl1a morphants. (D) gad1b expression is unaffected in the telencephalon, but reduced in the prethalamus and hypothalamus. (E) Tg(dlx1a/2aIG:GFP) embryos reduced gfp mRNA expression in the prethalamus of ascl1a morphants but telencephalic expression appears unaffected. (F) Tg(dlx1URE2:GFP) embryos show reduced gfp mRNA expression in the telencephalon and prethalamus. (G) The loss of GFP expression in the Tg(dlx5a/6a:GFP) transgenic line is consistent with a loss of dlx5a expression. Dashed box represents prethalamus region. Tel, telencephalon; Pth, prethalamus; Hy, hypothalamus. Scale bar=50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx1a WT control Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
WT + MO2-ascl1a standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
dlx2a WT control Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
WT + MO2-ascl1a standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
dlx5a WT control Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
WT + MO2-ascl1a standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
gad1b WT control Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
WT + MO2-ascl1a standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
GFP ot1Tg standard conditions Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
ot1Tg + MO2-ascl1a standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
ot2Tg standard conditions Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
ot2Tg + MO2-ascl1a standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
ot4Tg standard conditions Prim-5 hypothalamus IFL
Prim-5 telencephalon IFL
Prim-5 ventral thalamus IFL
ot4Tg + MO2-ascl1a standard conditions Prim-5 telencephalon IFL
Prim-5 ventral thalamus IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 381(1), MacDonald, R.B., Pollack, J.N., Debiais-Thibaud, M., Heude, E., Talbot, J.C., and Ekker, M., The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon, 276-85, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.