|ZFIN ID: ZDB-FIG-131017-8|
Fig. 2 Ascl1a function is required for proper expression of the dlx and gad1b genes in the forebrain at 24 hpf. (A, B) Expression of dlx1a and dlx2a is reduced in the prethalamus and hypothalamus (asterisk), but not affected in the telencephalon of ascl1a morphants compared to control embryos. (C) The expression of dlx5a is particularly reduced in the prethalamus and hypothalamus in ascl1a morphants. (D) gad1b expression is unaffected in the telencephalon, but reduced in the prethalamus and hypothalamus. (E) Tg(dlx1a/2aIG:GFP) embryos reduced gfp mRNA expression in the prethalamus of ascl1a morphants but telencephalic expression appears unaffected. (F) Tg(dlx1URE2:GFP) embryos show reduced gfp mRNA expression in the telencephalon and prethalamus. (G) The loss of GFP expression in the Tg(dlx5a/6a:GFP) transgenic line is consistent with a loss of dlx5a expression. Dashed box represents prethalamus region. Tel, telencephalon; Pth, prethalamus; Hy, hypothalamus. Scale bar=50 μm.
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Reprinted from Developmental Biology, 381(1), MacDonald, R.B., Pollack, J.N., Debiais-Thibaud, M., Heude, E., Talbot, J.C., and Ekker, M., The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon, 276-85, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.