ZFIN Gene: ascl1b

ZFIN ID: ZDB-GENE-980526-174

ZFIN ID: ZDB-GENE-980526-174

Gene Name:

achaete-scute family bHLH transcription factor 1b

Gene Symbol:

ascl1b Nomenclature History

Previous Names:

ashb, Zash-1b (1), zashb

Gene Type:

protein_coding_gene

Location:

Chr: 7 Mapping Details/Browsers

Description:

Predicted to contribute to DNA-binding transcription factor activity. Involved in chordate embryonic development; endocrine pancreas development; and hindbrain morphogenesis. Predicted to localize to RNA polymerase II transcription regulator complex. Is expressed in several structures, including central nervous system; neural plate; neural tube; neurons; and posterior neural keel. Human ortholog(s) of this gene implicated in congenital central hypoventilation syndrome. Orthologous to several human genes including ASCL2 (achaete-scute family bHLH transcription factor 2).

Genome Resources:

Alliance (1), Gene:30478 (1), Ensembl(GRCz11):ENSDARG00000101628 (1)

GENE EXPRESSION
All Expression Data:	62 figures from 36 publications
Directly Submitted Expression Data:	6 figures (15 images) from Thisse et al., 2005 [eu104]
Wild-type Stages, Structures:	Gastrula:Bud (10.0h-10.33h) to Adult (90d-730d, breeding adult)
	basal plate midbrain region brain neoplasm brain neuroblast (sensu Vertebrata) brain caudal tuberal nucleus central nervous system neuronal stem cell central nervous system cerebellum diencephalon forebrain head hindbrain neuroblast (sensu Vertebrata) hindbrain posterior region hindbrain ventral region hindbrain hindbrain neural plate hypothalamus midbrain neuroblast (sensu Vertebrata) midbrain ventral region midbrain nervous system neural plate neural rod neural tube neuroblast optic tectum pancreas primordium posterior recess presumptive rhombomere 2 presumptive rhombomere 4 presumptive telencephalon dorsal region presumptive telencephalon pretectum primary neuron hindbrain rhombomere compartment boundary rhombomere rhombomere 2 dorsal region rhombomere 2 ventral region rhombomere 2 rhombomere 3 rhombomere 4 dorsal region rhombomere 4 ventral region rhombomere 4 rhombomere 5 rhombomere 6 serotonergic neuron spinal cord spinal cord neural keel spinal cord neural tube tegmentum ventral-most region tegmentum telencephalon third ventricle medial region trunk embryonic structure unspecified ventricular zone whole organism
High Throughput Expression:	GEO (1) Expression Atlas (1)

MUTATIONS AND SEQUENCE TARGETING REAGENTS

Allele	Type	Localization	Consequence	Mutagen	Suppliers
sa38638	Point Mutation	Unknown	Premature Stop	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)
sa41003	Point Mutation	Unknown	Splice Site	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)
ulg002	Small Deletion	Unknown	Frameshift	CRISPR

Sequence Targeting Reagents

Targeting Reagent	Created Alleles	Publications
CRISPR1-ascl1b	ulg002	1
MO1-ascl1b	N/A	6

PHENOTYPE

Data:	Fig. S3 from Ghaye et al., 2015
Observed in:	pancreatic D cell somatostatin secreting cell

DISEASE ASSOCIATED WITH ascl1b HUMAN ORTHOLOG

Disease Ontology Term	Multi-Species Data	OMIM Term	OMIM Phenotype ID
congenital central hypoventilation syndrome	Alliance	Central hypoventilation syndrome, congenital	209880
		Haddad syndrome	209880

DISEASE ASSOCIATED WITH ascl1b VIA EXPERIMENTAL MODELS No data available

GENE ONTOLOGY

Ontology	GO Term
Biological Process	hindbrain morphogenesis (more)
Cellular Component	nucleus (more)
Molecular Function	DNA-binding transcription factor activity (more)

GO Terms (all 24)

PROTEIN FAMILIES, DOMAINS AND SITES No links to external sites available

TRANSCRIPTS
Type	Name	Length (nt)	Analysis
mRNA	ascl1b-203 (1)	1273	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT

Browsers: UCSC, NCBI, Ensembl, ZFIN

INTERACTIONS AND PATHWAYS

ANTIBODIES No data available

PLASMIDS No data available

CONSTRUCTS WITH SEQUENCES FROM ascl1b

Construct	Regulatory Regions	Coding Sequences	Species	Tg Lines	Publications
TgBAC(ascl1b:EGFP-2A-Cre-ERT2)	ascl1b	2A, Cre, EGFP, ERT2	Danio rerio	1	1

MARKER RELATIONSHIPS

ascl1b Contained in:	[BAC] CH211-210P4 (1)
ascl1b Encodes:	[EST] eu104 (1) [cDNA] MGC:85958 (1)

SEQUENCE INFORMATION

Type	Accession #	Length (nt/aa)	Analysis
RNA	RefSeq:NM_131231 (1)	1248 nt	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Genomic	GenBank:BX088691 (1)	150021 nt	Select Tool MegaBLAST Ensembl
Polypeptide	UniProtKB:A0A2R8S0I4 (1)	863 aa	Select Tool ZFIN BLAST SIB BLAST Ensembl Protein Blast

Sequence Information (all 13)

ORTHOLOGY for ascl1b ( Chr: 7 )

CITATIONS (93)

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