ZFIN ID: ZDB-FIG-130801-4
Teslaa et al., 2013 - Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development. Developmental Biology   380(1):73-86 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 5-9 somites

Fig. 4 Zic2b promotes timely neural crest induction. (A–L) Wild-type embryos stained by two-color ISH for expression of dlx3b, foxd3, zic2a and zic2b. (A,B) At 70% epiboly, weak expression of dlx3b (orange) and zic2a (purple) form two adjacent domains. (C,D) These domains are stronger by 90% epiboly but continue to overlap, suggesting the neural plate border is not completely formed. (E,F) At 1S, dlx3b (orange) and zic2a (purple) begin to separate. (G,H) Foxd3 (orange) straddles the border beginning at 1S, overlapping neural and non-neural (dlx3b, purple) markers. (I) At 1S, NC marker foxd3 (orange) and zic2b (purple) overlap at the neural plate border. (J,K) From 3-6S, zic2b (orange) is expressed in two anterior, bilateral domains from which zic2a (purple) is excluded. (L) Zic2b expression at the neural plate border extends into the posterior neural keel. (M,N) Sna1b expression is unchanged in zic2a morphants during early somitogenesis (37/48, 5 exp.). (O) Zic2b morphants have reduced (19/47, 5 exp.) or absent (16/47, 5 exp.) NC domains. (P) The sna1b domain is reduced (11/38, 5 exp.) or more frequently absent (24/38, 5 exp.) in double zic2 morphants. (A,C,E and G) are lateral views, anterior to the right. (B, D, F and H) are high magnification images of (A, C, E and G), respectively. (I–P) are dorsal views with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT standard conditions Shield presumptive ectoderm ISH
90%-epiboly non neural ectoderm ISH
1-4 somites non neural ectoderm ISH
foxd3 WT standard conditions 1-4 somites neural crest ISH
snai1b WT control 5-9 somites neural crest ISH
WT + MO2-zic2b standard conditions 5-9 somites neural crest ISH
WT + MO2-zic2b + MO3-zic2a standard conditions 5-9 somites neural crest ISH
WT + MO3-zic2a standard conditions 5-9 somites neural crest ISH
zic2a WT standard conditions Shield anterior presumptive neural plate ISH
90%-epiboly anterior neural plate lateral region ISH
1-4 somites anterior neural keel lateral region ISH
1-4 somites cranial neural crest ISH
5-9 somites anterior neural rod lateral region ISH
5-9 somites Not Detected posterior neural rod lateral region ISH
zic2b WT standard conditions 1-4 somites anterior neural keel lateral region ISH
1-4 somites cranial neural crest ISH
5-9 somites anterior neural rod lateral region ISH
5-9 somites cranial neural crest ISH
5-9 somites posterior neural rod lateral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-zic2b standard conditions 5-9 somites neural crest cell cellular quality, abnormal
5-9 somites neural crest cell differentiation decreased process quality, abnormal
WT + MO2-zic2b + MO3-zic2a standard conditions 5-9 somites neural crest cell cellular quality, abnormal
5-9 somites neural crest cell differentiation decreased process quality, abnormal
WT + MO3-zic2a standard conditions 5-9 somites neural crest cell differentiation process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 380(1), Teslaa, J.J., Keller, A.N., Nyholm, M.K., and Grinblat, Y., Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development, 73-86, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.