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General Information
ZIRC
ZFIN ID: ZDB-FIG-120905-38
Bräutigam et al., 2011 - Vertebrate-specific glutaredoxin is essential for brain development. Proceedings of the National Academy of Sciences of the United States of America   108(51):20532-7 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 2

Knockdown of zfGrx2 suppressed formation of a neuronal network. Morpholino-induced knockdown of zfGrx2 led to loss of early HuC/D positive neurons between 22 and 24 hpf (A). As demonstrated by different techniques, all tested neuronal subgroups were affected at 24 hpf: glutamatergic excitatory interneurons, dopaminergic neurons, and secondary motor neurons (B). Embryos lacking zfGrx2 were not able to develop an axonal scaffold (C). Ac, anterior commissure; dlt, dorsal longitudinal tract; nMLF, nucleus of the medial longitudinal fascicle; poc, posterior commissure; tg, trigeminal ganglion; vlt, ventral longitudinal tract. Scale bar, 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nns1Tg control 14-19 somites spinal cord neural tube neuron IFL
nns1Tg + MO1-glrx2 standard conditions 14-19 somites spinal cord neural tube neuron IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC WT control Prim-5 secondary motor neuron
IHC WT + MO1-glrx2 standard conditions Prim-5 secondary motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-glrx2 standard conditions 14-19 somites - 26+ somites central nervous system has fewer parts of type white matter, abnormal
14-19 somites - 26+ somites central nervous system development disrupted, abnormal
14-19 somites - 26+ somites central nervous system projection neuron axonogenesis disrupted, abnormal
14-19 somites - 26+ somites neuron amount, normal
14-19 somites - 26+ somites neuron spatial pattern, normal
Prim-5 central nervous system has fewer parts of type white matter, abnormal
Prim-5 central nervous system development disrupted, abnormal
Prim-5 central nervous system projection neuron axonogenesis disrupted, abnormal
Prim-5 dopaminergic neuron decreased amount, abnormal
Prim-5 glutamatergic neuron decreased amount, abnormal
Prim-5 neuron decreased amount, abnormal
Prim-5 secondary motor neuron decreased amount, abnormal
nns1Tg + MO1-glrx2 standard conditions Prim-5 neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA