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ZIRC
ZFIN ID: ZDB-FIG-120905-39
Bräutigam et al., 2011 - Vertebrate-specific glutaredoxin is essential for brain development. Proceedings of the National Academy of Sciences of the United States of America   108(51):20532-7 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 3

Outgrowth of neurites depends on the presence of Grx2. (A) Length and number of branching points of axons in embryos with silenced zfGrx2 expression were dramatically reduced 24 hpf, as demonstrated by immunohistochemistry using antiacetylated tubulin antibodies (asterisks mark cell bodies). (B) Quantitative data obtained from A. White bars, wild-type; black bars, zfGrx2 knockdown (mean ± SEM, n = 25, two-tailed Student’s t test, *** p < 3 × 10-7). (C) Three-dimensional reconstruction of the cytoskeleton (red, DNA; blue, tubulin; green, actin; single confocal layers as Insets; blue, DNA; yellow, actin) of SH-SY5Y cells grown for 8 d +/- RA. (D) Quantification of axon lengths (RA, n = 247; +RA, n = 156) and branching points (RA, n = 106; +RA, n = 213) of three independent experiments corresponding to C; white bars, control cells; black bars, hGrx2c+ cells (mean ± SEM, two-tailed Student’s t test, ***p < 0.008).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-glrx2 standard conditions Prim-5 central nervous system projection neuron axonogenesis disrupted, abnormal
Prim-5 neuron axon decreased branchiness, abnormal
Prim-5 neuron axon decreased length, abnormal
Prim-5 neuron projection development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA