ZFIN ID: ZDB-FIG-120320-4
Takesono et al., 2012 - Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo. Proceedings of the National Academy of Sciences of the United States of America   109(9):3371-3376 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Dome to 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 75%-epiboly to Prim-5

Fig. 1

Zebrafish slc3a2 is expressed specifically in the YSL and is essential for embryo development. (A) Expression of zebrafish slc3a2-a and slc3a3-b at different embryonic stages. Intensive slc3a2-a and slc3a2-b expression was observed around the YSN (arrowheads). (B) slc3a2 knockdown (MOs) causes a small head and short body axis at 24 hpf. Shown are examples of mild and severe morphological phenotypes. (C) Morphology at 24 hpf was categorized as normal (white), mild (light gray), or severe (dark gray) phenotypes presenting a small head and short body axis or as dead (black). Data shown are from three experiments; the total number of embryos is shown below each bar. (D) slc3a2 knockdown induces severe epiboly delay. Whole-mount in situ analyses for sox3, no-tail (ntl), and sox17 at the late gastrula stage are shown. The distance between the animal pole and the blastoderm margin is indicated by a black bracket.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
slc3a2a WT standard conditions Dome Not Detected whole organism ISH
Shield Not Detected whole organism ISH
75%-epiboly yolk syncytial layer ISH
1-4 somites yolk syncytial layer ISH
slc3a2b WT standard conditions Dome yolk syncytial layer ISH
Shield yolk syncytial layer ISH
90%-epiboly yolk syncytial layer ISH
1-4 somites yolk syncytial layer ISH
sox3 WT control 75%-epiboly to 90%-epiboly neuroectoderm ISH
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions 75%-epiboly to 90%-epiboly neuroectoderm ISH
sox17 WT control 75%-epiboly to 90%-epiboly endoderm ISH
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions 75%-epiboly to 90%-epiboly endoderm ISH
tbxta WT control 75%-epiboly to 90%-epiboly mesoderm ISH
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions 75%-epiboly to 90%-epiboly mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions 75%-epiboly - 90%-epiboly epiboly involved in gastrulation with mouth forming second delayed, abnormal
Prim-5 head decreased size, abnormal
Prim-5 post-vent region decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA